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microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1

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Abstract

MicroRNAs (miRNAs) play essential roles in muscle cell proliferation and differentiation. The muscle-specific miRNAs miR-1 and miR-206 have been shown to regulate muscle development and promote myogenic differentiation; however, it is likely that a number of other miRNAs play important roles in regulating myogenesis as well. microRNA-128 (miR-128) has been reported to be highly expressed in brain and skeletal muscle, and we found that miR-128 is also up-regulated during bovine skeletal muscle satellite cell differentiation using microarray analysis and qRT-PCR. However, little is known about the functions of miR-128 in bovine skeletal muscle satellite cell development. In this study, we investigated the biological functions of miR-128 in bovine skeletal muscle cell development. Using a dual-luciferase reporter assay, we confirmed that miR-128 regulates the Sp1 gene. Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. Inhibition of miR-128 increased Sp1 protein levels and promoted muscle satellite cell differentiation but also suppressed proliferation. Changes in miR-128 and Sp1 expression levels also affected the protein levels of MyoD and CDKN1A. Sp1, an activator of MyoD and a suppressor of CDKN1A, plays an important role in bovine muscle cell proliferation and differentiation. The results of our study reveal a mechanism by which miR-128 regulates bovine skeletal muscle satellite cell proliferation and myogenic differentiation via Sp1.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31201021), Natural Science Foundation of Tianjin (13JCQNJC14600), the Open Subjects for the Major Basic Research Program of Science and Technology Department of Inner Mongolia Autonomous Region (20130902), the Excellent Young Teachers Program of Tianjin (J010070514), and the Second-level Candidates Training Project of 131 Innovative Talents of Tianjin (J01005021104).

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    Authors and Affiliations

    1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

      Yang Dai, Wei Ran Zhang, Yi Min Wang, Xin Feng Liu, Xin Li, Xiang Bin Ding & Hong Guo

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    1. Yang Dai
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    2. Wei Ran Zhang
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    3. Yi Min Wang
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    4. Xin Feng Liu
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    5. Xin Li
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    Correspondence to Xiang Bin Ding or Hong Guo.

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    Yang Dai and Wei Ran Zhang contributed equally to this work.

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    Dai, Y., Zhang, W.R., Wang, Y.M. et al. microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1. Mol Cell Biochem 414, 37–46 (2016). https://doi.org/10.1007/s11010-016-2656-7

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