Crosstalk between TGF-β/Smad3 and BMP/BMPR2 signaling pathways via miR-17–92 cluster in carotid artery restenosis
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- Luo, T., Cui, S., Bian, C. et al. Mol Cell Biochem (2014) 389: 169. doi:10.1007/s11010-013-1938-6
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In the recent decades, carotid angioplasty and stenting (CAS) has been developed into a credible option for the patients with carotid stenosis. However, restenosis remains a severe and unsolved issue after CAS treatment. Restenosis is characterized by neointimal hyperplasia, which is partially caused by vascular smooth muscle cells (VSMC) proliferation. However, the molecular mechanism involved in the restenosis is still unclear. In this study, we demonstrated a functional crosstalk between two TGF-β superfamily signaling pathway members, Smad3 and BMPR2, in VSMC proliferation. Smad3 plays an important role in the TGF-β/Smad3 signaling pathway, and is significantly upregulated in the carotid artery with restenosis to promote VSMC proliferation. In contrast, BMP receptor II (BMPR2), an inhibitor of VSMC proliferation is downregulated in carotid restenosis. We further found that BMPR2 downregulation is mediated by miR-17–92 cluster, which is transcriptionally regulated by Smad3. Thus, Smad3 upregulation and Smad3/miR-17–92 cluster-dependent BMPR2 downregulation are likely to promote VSMC proliferation and restenosis. Taken together, our results may provide novel clues for early diagnosis of carotid restenosis and developing new therapeutic strategy.
KeywordsCarotid artery restenosisSmad3BMPR2miR-17–92 cluster
Carotid stenosis is a narrowing or constriction of the inner surface of the carotid artery [1, 2]. It is a major risk factor for stroke that leads to brain damage [3, 4]. Carotid stenosis is usually caused by atherosclerosis [5, 6], characterized by the atherosclerostic plagues accumulating in the artery wall, thus occluding the blood flow. In the last decade, carotid angioplasty and stenting (CAS) has been developed into a credible alternative treatment to carotid endarterectomy (CEA) for the patients with symptomatic moderate- and high-grade stenosis [7, 8]. However, restenosis still remains an unsolved issue following CAS treatment, and the restenosis rate is even higher than that following CEA treatment [9, 10]. Restenosis is a healing process of the arterial wall in response to mechanical injury caused by CAS and comprises two major processes—neointimal hyperplasia and vessel remodeling [11, 12]. Thus, to develop more effective therapeutic approaches for preventing restenosis, a better understanding of the molecular mechanism of restenosis is important.
One of the important characters of restenosis is vascular smooth muscle cells (VSMC) proliferation, which is likely to be mediated by transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) signaling pathway. TGF-β is known to play an important role in the development of restenosis [13, 14]. As a profibrotic cytokine, TGF-β enhances neointimal formation by stimulating extracellular matrix (ECM) synthesis [15, 16]. Among the many downstream effectors of TGF-β, Smad3 might play a critical role in the formation of intimal hyperplasia. It has been shown that TGF-β1, through Smad3-dependent pathway, stimulates VSMC fibronectin synthesis, thus enhancing neointimal ECM accumulation . Moreover, it has been shown that Smad3 overexpression also enhanced intimal hyperplasia via the stimulation of VSMC proliferation [13, 15]. Smad3 acts as a transcription factor that regulates the expression of a number of genes in the cellular processes [18, 19]. However, how Smad3 regulates in the carotid artery restenosis is largely unknown.
Besides TGF-β, bone morphogenetic protein (BMP) is another important member of the TGF-β superfamily [20, 21]. To date, around 20 BMP family members have been identified and characterized . BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes . Three type I receptors have been shown to bind BMP ligands, type IA and IB BMP receptors (BMPR1A and BMPR1B), and type IA activin receptor (ActR1A) . Three type II receptors for BMPs have also been identified, including type II BMP receptor (BMPR2), and type II and IIB activin receptors (ActR2 and ActR2B) . The function of BMP-dependent signaling pathway in carotid artery restenosis is not clear. However, loss-of-function mutations of BMP receptor II (BMPR2) are often identified in the patients with familial pulmonary arterial hypertension (PAH) [24, 25], a disease characterized by excessive VSMC proliferation, particularly in response to TGF-β . Thus, BMPR2 is likely to act as a negative regulator to suppress TGF-β-dependent VSMC proliferation. Since VSMC proliferation causes neointimal formation in carotid restenosis [11, 12], we hypothesize that Smad3-dependent signaling pathway has functional crosstalk with BMPR2 in VSMC proliferation during carotid restenosis.
Using human tissue samples, we confirmed that Smad3 is significantly upregulated in carotid restenosis. Moreover, we found that the expression level of BMPR2 was significantly reduced in human carotid artery restenosis. The ChIP analyses showed that BMPR2 was targeted by miR-17–92 cluster members, which is controlled by Smad3. Thus, our results reveal a novel crosstalk between Smad3 and BMPR2 through miR-17–92 cluster in carotid artery restenosis. This study may provide a novel therapeutic strategy for carotid artery restenosis, namely, inhibition of miR-17–92 cluster, which may not only block the Smad3 function but also rescue BMPR2 expression to relieve the neointimal formation.
Materials and methods
The carotid artery tissues were surgically removed from the patients with carotid restenosis following CAS treatment at Beijing Xuanwu Hospital in China, and were harvested for this study in accordance with informed consent principles.
Peripheral blood samples were collected from the patients with carotid restenosis following CAS treatment at Beijing Xuanwu Hospital in China after informed consent from the subjects. Peripheral blood samples from healthy volunteers were also collected as control. All the samples used in this study were harvested after obtaining approval from the ethics committees at Capital Medical University of China and University of Michigan.
Human carotid artery smooth muscle cells (hHCtASMCs) were obtained commercially (Cell Applications, Inc.) and cultured in Medium 231 supplemented with Smooth Muscle Growth Supplement (Life technologies) in 37 °C with 5 % CO2.
Histopathology and immunofluorescent staining
The artery specimens were fixed in 4 % paraformaldehyde for 24 h, dehydrated, and embedded into paraffin wax, then sectioned to 4 μm slides and processed for H&E staining.
To perform immunofluorescent staining, sections were deparaffinized and incubated with rabbit anti-Smad3 (1:500) (Upstate, Cat. # 04-1035) and mouse anti-BMPR2 (1:500) (Millipore, Cat. # MABD171). The slides were then washed in PBS and incubated with FITC-coupled secondary antibodies (1:1000, Jackson ImmunoResearch Laboratories).
Transfection of siRNAs to hHCtASMCs
hHCtASMCs were transfected with 500 ng of Smad3 siRNA and Mock siRNAs using a Primary P1 Nucleofection Kit and 4D Nucleofector machine (Lonza). Smad3 siRNA sequence was ordered from Dharmacon. The siRNA sense sequence is 5′-GAGAAACCAGUGACCACCA-3′, and the mock siRNA sense sequence is 5′-CUACAACUCCCACAACGUA-3′.
RNA isolation and quantitative reverse transcription polymerase chain reaction (qRT-PCR)
Total RNA was isolated from the carotid artery tissue using Trizol (Invitrogen) according to the manufacturer’s instruction. For circulating RNA isolation, the whole blood was allowed to stand overnight at 4 °C, and total circulating RNA was isolated from 200 μl serum using miRNeasy mini kit according to the manufacturer’s protocol (Qiagen).
Approximately, 500 ng of total RNA was reverse transcribed using oligo-dT primers. The cDNA was utilized as a template to amplify target genes with SYBR Premix Ex Tag Kit (TaKaRa). Specific primers were listed in Supplementary Table 1. Each RNA sample was evaluated in triplicate. Gene expression results were analyzed by the ΔΔCt method and normalized to β-actin expression. The qRT-PCR assay was performed on Bio-Rad iQ5 instrument. The data were analyzed using Optical System Software 2.0.
The expression levels of mature miRNAs were determined using miRNA-specific qRT-PCR. The specific RT and PCR primers for miR-17, 18a, 19a, 20a, 92a, 130a, 21, and U6 were purchased from Qiagen. Reverse transcription of miRNAs was performed with a miScript Reverse Transcription Kit (QiAGEN). The qPCR was performed on Bio-Rad IQ 5 instrument by means of SYBR Premix Ex Tag Kit (TaKaRa). The expression levels were normalized to the U6 endogenous control and measured by the comparative Ct (ΔΔCt) method.
Dual luciferase reporter gene construct and dual luciferase reporter assay
The target site of microRNA with ~60 flanking sequence in BMPR2 3′ UTR was synthesized with cleavage sites for Xba I (5′ end) and Not I (3′ end), and a mutated sequence of the binding site was also synthesized. The two constructs were termed as WT and Mut. The DNA oligos were cloned into the pRL-TK vector (Promega). The DNA sequences containing the miR-17–92 cluster, miR-130a, and miR-21 target sites as well as the mutant are shown in Supplementary Table 1. Each vector, along with 100 ng of PGL3 and 200 nmol/L miR-17–92 cluster, miR-130 and miR-21 or mimic control, was transfected into 293T cells. Cells were assayed for renilla and firefly luciferase activity using the Dual-Luciferase Reporter System (Promega).
Chromatin immunoprecipitation assay
Chromatin immunoprecipitation assays (ChIP) for artery tissues were performed according to the protocol described by Upstate. In brief, 30 mg of fresh artery tissues per antibody in every ChIP were chopped into small pieces and transferred into 10 mL PBS (plus protease inhibitor). The artery tissues were fixed by 1 % formaldehyde at room temperature for 15 min, and then stopped by adding fresh glycine to a final concentration of 0.125 M at temperature for 5 min. After being washed by cold PBS twice, the artery tissues were resuspended in 1 mL cold PBS (plus protease inhibitors). To achieve a single cell suspension, the Medimachine from Becton–Dickinson was used to grind tissue for 2 min. After centrifugation, the cell pellet was suspended in 6× volume of cell lysis buffer plus protease inhibitors for 15 min on ice, and lysed using a B dounce several times to aid in nuclei release. Cell nuclei were collected by centrifugation at 1,000×g at 4 °C. The cell nuclei were resuspended in 5 × volume of nuclei lysis buffer plus the protease inhibitors, incubated on ice for 20 min and then subjected to sonication. The artery tissue DNA was sonicated to an average size between 300 and 600 bp. Solubilized chromatin was immunoprecipitated with antibody against Smad3 (Upstate), and IgG was used as control. Antibody–chromatin complexes were pulled down using protein A-Sepharose. After crosslink reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol–chloroform, ethanol precipitated, and treated with RNase. ChIP DNA was subjected to PCR amplification using primers flanking the Smad3-binding site at the miRNA gene promoter.
Protein extraction and western blotting
Protein samples were extracted from the artery tissues by using total protein extraction kit (Millipore). The protein concentration was determined using BCA standard curve. Equal amounts of protein extracts were separated by SDS-PAGE and transferred electrophoretically to PVDF membranes (Millipore). The membranes were blocked in TBST containing 5 % milk at room temperature for 1 h. After washing with TBST, the blocked membranes were probed with rabbit anti-Smad3 (1:500) (Upstate), mouse anti-BMPR2 (1:500) (Millipore), and mouse anti-β-actin (1:10,000) (Sigma) primary antibodies overnight at 4 °C. After three consecutive 10-min washes with TBST, the membranes were incubated with HRP-conjugated goat-anti-rabbit or goat-anti-mouse secondary antibody for 1 h. The membranes were washed again three times with TBST and developed using the ECL+ detection system (GE Healthcare).
All the experiments were performed at least three times. Two-tailed Student’s t test was used for comparing the two groups. ANOVA was used for multiple-group (>2) comparison. Data were presented as mean ± SD p < 0.05 were considered statistically significant.
Result and discussion
TGF-β1/Smad3 signaling pathway has been shown to play a key role for promoting the neointimal formation during carotid restenosis, including VSMC proliferation, through experimental studies in the animal models [15, 16]. Thus, we examined the Smad3 expression levels in human carotid artery tissue with restenosis by immunofluorescence staining. As shown in Fig. 1b, Smad3 was positively stained in the neointima hyperplasia but not normal carotid artery. Moreover, we confirmed the upregulation of Smad3 using both RT-PCR and Western blotting (Fig. 1c, d). Taken together, these results suggest that the expression of Smad3 was significantly increased in the carotid restenosis.
Besides Smad3, BMPR2 also regulates VSMC proliferation. Thus, we questioned whether BMPR2 participated in developing carotid restenosis. Again, we examined the expression of BMPR2 in carotid restenosis. Both the immunofluorescence staining and Western blotting results indicated a significant decrease of the protein level of BMPR2 in carotid tissues with restenosis (Fig. 1b, c). With the quantitative analysis of Western blotting results, we found a ~80 % decrease of BMPR2 in carotid restenosis (Fig. 1e). However, the result of qRT-PCR showed a slight decrease of BMPR2 mRNA level in artery tissues with restenosis compared with that in normal adjacent artery tissues (Fig. 1f).
We next detected the expression level of miR-17, miR-19a, miR-20a, miR-92a as well as another miR-17–92 family member, miR-18a in the carotid artery tissues with restenosis. All these microRNAs were found to be significantly upregulated in carotid restenosis compared with that in adjacent normal artery (Fig. 2d). These results further support that miR-17–92 cluster members induce the carotid restenosis formation by suppressing the expression of BMPR2. Of note, as a member of miR-17–92 cluster, miR-18a was also upregulated in carotid artery restenosis although little evidence indicate that miR-18a is involved in artery restenosis. It is possible that the upregulation of miR-18a might be a byproduct of expressing miR-17–92 cluster in the carotid artery tissue with restenosis (Fig. 2d).
Recently, extracellular miRNAs have been found to circulate in the bloodstream, and the circulating miRNAs are remarkably stable [36–38]. Although the precise cellular release mechanism of miRNAs remains elusive, some studies revealed that these circulating miRNAs can be delivered to recipient cells, where they can regulate the translation of target genes [39, 40]. It raises the possibility that miRNAs can be probed in the blood and can serve as novel diagnostic markers [38, 41]. Thus, as a potential clinical application for the early diagnosis of the restenosis, we examined the serum levels of four circulating miR-17–92 cluster members. We isolated the serum microRNAs from eight patients with carotid restenosis following CAS as well as eight healthy volunteers. The qRT-PCR was performed to detect the circulating microRNA levels. The upregulation of miR-17–92 cluster members, including miR-17, miR-19a, miR-20a, and miR-92a, was clearly observed in the sera from the patients (Fig. 2e).
This study was supported by the National Institute of Health (CA132755 and CA130899 to X.Y.), the University of Michigan Cancer Center and GI Peptide Research Center. X.Y. is a recipient of the Era of Hope Scholar Award from the Department of Defense.