Molecular and Cellular Biochemistry

, Volume 372, Issue 1, pp 155–160

eNOS polymorphism associated with metabolic syndrome in children and adolescents

  • Josiane A. Miranda
  • Vanessa A. Belo
  • Débora C. Souza-Costa
  • Carla M. M. Lanna
  • Jose E. Tanus-Santos
Article

DOI: 10.1007/s11010-012-1456-y

Cite this article as:
Miranda, J.A., Belo, V.A., Souza-Costa, D.C. et al. Mol Cell Biochem (2013) 372: 155. doi:10.1007/s11010-012-1456-y

Abstract

We investigated whether genetic polymorphisms in the endothelial nitric oxide (eNOS) gene (T786C in the promoter region, Glu298Asp in exon 7, and 4b/4a in intron 4) or eNOS haplotypes are associated with metabolic syndrome (MetS) in obese children and adolescents. We studied 242 subjects: 108 healthy (controls), 64 normotensive obese, and 70 obese children and adolescents with MetS. Genotypes were determined by Taqman® allele discrimination assay and real-time polymerase chain reaction (PCR), and PCR followed by fragment separation by electrophoresis. We compared the distribution of eNOS genotypes, alleles, and haplotypes in the three groups of subjects. The CC genotype for the T786C polymorphism was more common in the MetS group than in the control group (OR = 3.27; CI 1.81–9.07; P < 0.05). However, we found no significant differences in the distribution of eNOS haplotypes (P > 0.00625; P for significance after correction for multiple comparisons). Our findings suggest that while eNOS haplotypes are not relevant, the CC genotype for the T786C polymorphism is associated with MetS in obese children and adolescents. Further studies examining interactions of eNOS haplotypes with environmental factors and other genetic markers are warranted.

Keywords

AdolescentsChildrenEndothelial nitric oxide synthaseObesityMetabolic syndromePolymorphismHaplotype

Supplementary material

11010_2012_1456_MOESM1_ESM.docx (33 kb)
Supplementary material 1 (DOCX 32 kb)

Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Josiane A. Miranda
    • 1
  • Vanessa A. Belo
    • 2
  • Débora C. Souza-Costa
    • 2
  • Carla M. M. Lanna
    • 3
  • Jose E. Tanus-Santos
    • 2
  1. 1.Department of PharmacologyState University of CampinasCampinasBrazil
  2. 2.Department of Pharmacology, Faculty of Medicine of Ribeirao PretoUniversity of Sao PauloRibeirão PrêtoBrazil
  3. 3.Department of Physiology, Institute of Biologic ScienceFederal University of Juiz de ForaJuiz de ForaBrazil