Molecular and Cellular Biochemistry

, Volume 369, Issue 1, pp 175–181

Analysis of the transcriptome of differentiating and non-differentiating preadipocytes from rats and humans by next generation sequencing

Authors

  • Fabian Birzele
    • Target Discovery ResearchBoehringer Ingelheim Pharma GmbH & Co. KG
  • Sybille Fässler
    • CardioMetabolic Diseases ResearchBoehringer Ingelheim Pharma GmbH & Co. KG
  • Heike Neubauer
    • CardioMetabolic Diseases ResearchBoehringer Ingelheim Pharma GmbH & Co. KG
  • Tobias Hildebrandt
    • Target Discovery ResearchBoehringer Ingelheim Pharma GmbH & Co. KG
    • CardioMetabolic Diseases ResearchBoehringer Ingelheim Pharma GmbH & Co. KG
Article

DOI: 10.1007/s11010-012-1380-1

Cite this article as:
Birzele, F., Fässler, S., Neubauer, H. et al. Mol Cell Biochem (2012) 369: 175. doi:10.1007/s11010-012-1380-1

Abstract

Alongside cell lines such as 3T3-L1 cells, primary cell culture models of adipogenesis have helped in developing an understanding of the process of adipocyte recruitment and maintenance, which may lead to therapeutic advances to treat the growing epidemic of obesity. Recently, it has been demonstrated that fat cell progenitors (DFAT) established through ceiling culture of adipocytes retain an enhanced ability to undergo adipocyte differentiation compared to preadipocytes isolated from the stromal vascular fraction of adipose tissue. Clonal expansion of rat DFAT cells identified differentiation capable and incapable cell strains. To understand the mechanisms underlying these differences, comparison of their transcriptomes by next generation sequencing was performed. Two hundred seventy-eight genes with a significant fold change of 1.4 were detected as being consistently deregulated between differentiating and non-differentiating strains. Bioinformatic network analyses identified components of the extra-cellular matrix and PPARγ as important genes in this process, suggesting crosstalk between ECM and transcription factors influences differentiation. Analyses of the transcriptomes of human DFAT cells in early and late passage (non-differentiating) confirmed the importance of these pathways in maintaining an adipogenic potential.

Keywords

PreadipocyteDifferentiationExtra-cellular matrixPPARγTranscriptome

Supplementary material

11010_2012_1380_MOESM1_ESM.docx (39 kb)
Supplementary material 1 (DOCX 38 kb)

Copyright information

© Springer Science+Business Media, LLC. 2012