Skip to main content
SpringerLink
Log in

Anti-inflammatory and anti-apoptotic effects of Crataegus oxyacantha on isoproterenol-induced myocardial damage

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

This study was designed to evaluate the anti-inflammatory and anti-apoptotic effects of the alcoholic extract of the berries of Crataegus oxyacantha (AEC), a medicinal herb, on isoproterenol-induced myocardial infarction (MI) in a rat model. Three groups of Wistar albino rats, each comprising six animals, were selected for this study. Group I rats served as control. Group II rats were given isoproterenol (85 mg/kg body weight) subcutaneously on 59th and 60th days. Group III rats were given AEC (0.5 ml/100 g body weight/day), orally on a daily basis for 60 days, and isoproterenol (85 mg/kg body weight, subcutaneously) was given on 59th and 60th days. On the 61st day, the animals were sacrificed, and marker enzymes like lactate dehydrogenase (LDH) and creatine kinase (CK) were estimated in serum. In the heart tissue sample, antioxidant status, lipid peroxidation and anti-inflammatory properties of AEC were determined. Isoproterenol significantly increased the release of LDH, CK in serum, decreased the antioxidant status in the heart along with an increase in lipid peroxidation. Nitritive stress and apoptosis were seen in isoproterenol-induced rat heart. Pre-treatment with the AEC for 60 days had a significant effect on all the above factors and maintained near normal status. The study confirms the protective effect of AEC against isoproterenol-induced inflammation and apoptosis-associated MI in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Taimor G, Hofstaetter B, Piper HM (2000) Apoptosis induction by nitric oxide in adult cardiomyocytes via cGMP-signaling and its impairment after simulated ischemia. Cardiovasc Res 45(3):588–594

    Article  PubMed  CAS  Google Scholar 

  2. World Health Organisation (2006) Annex 2: death by cause, sex and mortality stratum in WHO regions, estimates for 2002. World Health Organisation, Geneva

  3. Levy RI, Feinleib M (1984) Risk factors for coronary artery disease and their management. In: Brawnwald E (ed) Heart disease. A textbook of cardiovascular medicine Vol 2, 2nd edn. WB Saunders, Philadelphia, pp 1205–1234

    Google Scholar 

  4. Rajadurai M, Prince PS (2005) Comparative effects of Aegle marmelos extract and alpha-tocopherol on serum lipids, lipid peroxides and cardiac enzyme levels in rats with isoproterenol-induced myocardial infarction. Singapore Med J 46:78–81

    PubMed  CAS  Google Scholar 

  5. Song YH, Cai H, Gu N, Qian CF, Cao SP, Zhao ZM (2011) Icariin attenuates cardiac remodelling through down-regulating myocardial apoptosis and matrix metalloproteinase activity in rats with congestive heart failure. J Pharm Pharmacol 63(4):541–549

    Article  PubMed  CAS  Google Scholar 

  6. Sasikumar CS, Devi CS (2000) Effect of abana an ayurvedic formulation, on lipid peroxidation in experimental myocardial infarction in rats. Indian J Exp Biol 38(8):827–830

    PubMed  CAS  Google Scholar 

  7. Shanthi S, Parasakthy K, Deepalakshmi PD, Devaraj SN (1994) Hypolipdemic activity of tincture of Crataegus in rats. Indian J Biochem Biophys 31:143–146

    PubMed  CAS  Google Scholar 

  8. Karthikeyan K, Bai BR, Devaraj SN (2007) Efficacy of grape seed proanthocyanidins on serum and heart tissue lipids in rats subjected to isoproterenol-induced myocardial injury. Vascul Pharmacol 47(5–6):295–301

    Article  PubMed  CAS  Google Scholar 

  9. King J (1965) The dehydrogenases or oxidoreductases, Lactate dehydrogenase in practical clinical enzymology. Van D Nostrand, London 83–93

  10. Okinaka S, Kumagai H, Ebashi S, Sugita H, Momoi H, Toyokura Y, Fujie Y (1961) Serum creatine phosphokinase. Activity in progressive muscular dystrophy and neuromuscular diseases. Arch Neurol 4:520–525

    Article  PubMed  CAS  Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265

    PubMed  CAS  Google Scholar 

  12. Weydert CJ, Cullen JJ (2010) Measuremnet of superoxide dismutase, catalase and glutathione peroxidise in cultured cells and tissue. Nat Protoc 5(1):51–66 (Epub 2009 Dec 17)

    Article  PubMed  CAS  Google Scholar 

  13. Higuchi Y, Linn S (1995) Purification of all forms of HeLa cell mitochondrial DNA and assessment of damage to it caused by hydrogen peroxide treatment of mitochondria or cells. J Biol Chem 270(14):7950–7956

    Article  PubMed  CAS  Google Scholar 

  14. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–315

    Article  PubMed  CAS  Google Scholar 

  15. Pearl W, Cascarano J, Zweifach BW (1963) Microdetermination of cytochrome oxidase in rat tissues by the oxidation of N-phenyl-p-phenylenediamine or ascorbic acid. J Histochem Cytochem 11:102. doi:10.1177/11.1.102

    Article  CAS  Google Scholar 

  16. Moshage H, Kok B, Huizenga JR, Jansen PL (1995) Nitrite and nitrate determination in plasma: a critical evaluation. Clin Chem 41:892–896

    PubMed  CAS  Google Scholar 

  17. Gill C, Mestril R, Samali A (2002) Losing heart: the role of apoptosis in heart disease—a novel therapeutic target? FASEB J 16(2):135–146

    Article  PubMed  CAS  Google Scholar 

  18. Rigelsky JM, Sweet BV (2002) Hawthorn: pharmacology and therapeutic uses. Am J Health Syst Pharm 59(5):417–422

    PubMed  Google Scholar 

  19. Rembold CM, Chen XL (1998) The buffer barrier hypothesis, [Ca2+]i homogeneity, and sarcoplasmic reticulum function in swine carotid artery. J Physiol 513(Pt 2):477–492

    Article  PubMed  CAS  Google Scholar 

  20. Brawley L, Shaw AM, MacDonald A (2000) Role of endothelium/nitric oxide in a typical beta-adrenoceptor-mediated relaxation in rat isolated aorta. Eur J Pharmacol 398(2):285–296

    Article  PubMed  CAS  Google Scholar 

  21. Ferro A, Queen LR, Priest RM, Xu B, Ritter JM, Poston L, Ward JP (1999) Activation of nitric oxide synthase by beta 2-adrenoceptors in human umbilical vein endothelium in vitro. Br J Pharmacol 126(8):1872–1880

    Article  PubMed  CAS  Google Scholar 

  22. Davel AP, Kawamoto EM, Scavone C, Vassallo DV, Rossoni LV (2006) Changes in vascular reactivity following administration of isoproterenol for 1 week: a role for endothelial modulation. Br J Pharmacol 148(5):629–639

    Article  PubMed  CAS  Google Scholar 

  23. Jayalakshmi R, Niranjali Devaraj S (2004) Cardioprotective effect of tincture of Crataegus on isoproterenol-induced myocardial infarction in rats. J Pharm Pharmacol 56:921–926

    Article  PubMed  CAS  Google Scholar 

  24. Snyder CM, Shroff EH, Liu J, Chandel NS (2009) Nitric oxide induces cell death by regulating anti-apoptotic BCL-2 family members. PLoS One 4(9):e7059

    Article  PubMed  Google Scholar 

  25. Kim SF, Huri DA, Snyder SH (2005) Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science. doi:10.1126/science.1119407

  26. Hajjar DP, Lander HM, Pearce FS, Upmacis RK, Pomerantz KB (1995) Nitric oxide enhances prostaglandin-H synthase activity by a heme-independent mechanism: evidence implicating nitrosothiols. J Am Chem Soc 117:3340–3346

    Article  CAS  Google Scholar 

  27. Saito T, Rodger IW, Shennib H, Hu F, Tayara L, Giaid A (2003) Cyclooxygenase-2 (COX-2) in acute myocardial infarction: cellular expression and use of selective COX-2 inhibitor. Can J Physiol Pharmacol 81(2):114–119

    Article  PubMed  CAS  Google Scholar 

  28. Zhang Z, Vezza R, Plappert T, McNamara P, Lawson JA, Austin S, Praticò D, Sutton MS, Fitz Gerald GA (2003) COX-2-dependent cardiac failure in Gh/tTG transgenic mice. Circ Res 92(10):1153–1161

    Article  PubMed  CAS  Google Scholar 

  29. Banerjee SK, Sood S, Dinda AK, Das TK, Maulik SK (2003) Chronic oral administration of raw garlic protects against isoproterenol-induced myocardial necrosis in rat. Comp Biochem Physiol C Toxicol Pharmacol 136(4):377–386

    Article  PubMed  CAS  Google Scholar 

  30. Badjatia N, Satyam A, Singh P, Seth A, Sharma A (2009) Altered antioxidant status and lipid peroxidation in Indian patients with urothelial bladder carcinoma. J Urol Oncol 28:1–8

    Google Scholar 

  31. Beckman JS, Koppenol WH (1996) Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly. Am J Physiol 271:C1424–C1437

    PubMed  CAS  Google Scholar 

  32. Virag L, Marmer DJ, Szabo C (1998) Crucial role of apopain in the peroxynirite-induced apoptotic DNA fragmentation. Free Radic boil Med 25:1075–1082

    Article  CAS  Google Scholar 

  33. Ushmorov A, Ratter F, Lehmann V (1999) Nitric-oxide-induced apoptosis in human leukemic lines requires mitochondrial lipid degradation and cytochrome c release. Blood 93:2342–2352

    PubMed  CAS  Google Scholar 

  34. MacMillan-Crow LA, Crow JP, Thompson JA (1998) Peroxynitrite-mediated inactivation of manganese superoxide dismutase involves nitration and oxidation of critical tyrosine residue. Biochemistry 37:1613–1622

    Article  PubMed  CAS  Google Scholar 

  35. Oyadomari S, Takeda K, Takiguchi M (2001) Nitric oxide-induced apoptosis in pancreatic beta cells is mediated by the endoplasmic reticulum stress pathway. Proc Natl Acad Sci USA 98:10845–10850

    Article  PubMed  CAS  Google Scholar 

  36. Jayachandran KS, Khan M, Selvendiran K, Devaraj SN, Kuppusamy P (2010) Crataegus oxyacantha extract attenuates apoptotic incidence in myocardial Ischemia–reperfusion injury by regulating AKT and HiF-1 signalling pathways. J. Cardiovascu pharmocol 56(5):526–531

    Article  CAS  Google Scholar 

  37. Hoffman DL (2006) Hawthorn (online 2006 October 9). Available from: URL: http://www.crystalgard-enherbs.com/HerbRefH.htm. Accessed October 9, 2006

  38. Thiruchenduran M, Vijayan NA, Sawaminathan JK, Devaraj SN (2010) Protective effect of grape seed proanthocyanidins against cholesterol cholic acid diet-induced hypercholesterolemia in rats. Cardiovasc Pathol 20(6):361–368

    Article  PubMed  Google Scholar 

  39. Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E (2007) Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappa B activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappa B activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm 2007:45673

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Madras, Guindy Campus, Chennai, 600025, Tamil Nadu, India

    Navin Alukkathara Vijayan, Mohana Thiruchenduran & Sivasitamparam Niranjali Devaraj

Authors
  1. Navin Alukkathara Vijayan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohana Thiruchenduran
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sivasitamparam Niranjali Devaraj
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sivasitamparam Niranjali Devaraj.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vijayan, N.A., Thiruchenduran, M. & Devaraj, S.N. Anti-inflammatory and anti-apoptotic effects of Crataegus oxyacantha on isoproterenol-induced myocardial damage. Mol Cell Biochem 367, 1–8 (2012). https://doi.org/10.1007/s11010-012-1251-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-012-1251-9

Keywords

Search

Navigation