Molecular and Cellular Biochemistry

, Volume 364, Issue 1, pp 243–251

Genome-wide analysis of HMGA2 transcription factor binding sites by ChIP on chip in gastric carcinoma cells

  • Lang Zha
  • Ziwei Wang
  • Weixue Tang
  • Neng Zhang
  • Gang Liao
  • Zhen Huang
Article

DOI: 10.1007/s11010-012-1224-z

Cite this article as:
Zha, L., Wang, Z., Tang, W. et al. Mol Cell Biochem (2012) 364: 243. doi:10.1007/s11010-012-1224-z

Abstract

High mobility group protein A2 (HMGA2) is an architectural transcription factor that plays an important role in development and progression of malignant neoplasias. Recently, some studies reported that HMGA2 is also implicated in epithelial–mesenchymal transitions (EMT) and cancer stem cells. But the underlying mechanisms of these conditions are poorly understood. Therefore, we established an EMT model of gastric carcinoma cells by overexpressing HMGA2 in vitro, then global mapping of HMGA2 potential transcription factor binding sites was identified by promoter microarray in these cells, and the date obtained from the microarrays were validated via chromatin immunoprecipitation–PCR (ChIP-PCR) and real-time PCR. HMGA2 potential target genes were classified in KEGG database and Gene Ontology (GO) analyses. To our knowledge, this is the first report on the genome-wide analysis of HMGA2 downstream direct targets, and these findings will be valuable in understanding the roles of HMGA2 in EMT.

Keywords

HMGA2Architectural transcription factorChIP on chip analysisGastric carcinomaEpithelial–mesenchymal transitionsKEGG databaseGene ontology

Supplementary material

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Supplementary material 1 (XLSX 48 kb)
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Supplementary material 2 (XLS 24 kb)
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Supplementary material 3 (XLSX 342 kb)

Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Lang Zha
    • 1
  • Ziwei Wang
    • 1
  • Weixue Tang
    • 2
  • Neng Zhang
    • 1
  • Gang Liao
    • 1
  • Zhen Huang
    • 1
  1. 1.Department of Gastrointestinal SurgeryFirst Affiliated Hospital of Chongqing Medical UniversityChongqingPeople’s Republic of China
  2. 2.Key Laboratory of General Surgery in ChongqingChongqingPeople’s Republic of China