Molecular and Cellular Biochemistry

, Volume 354, Issue 1, pp 67–75

Induction of pluripotent stem cells transplantation therapy for ischemic stroke

Authors

  • Mei Jiang
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Lei Lv
    • Departments of NeurologyHuashan Hospital, Fudan University
  • Haifeng Ji
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Xuelian Yang
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Wei Zhu
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Liying Cai
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Xiaju Gu
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Changfeng Chai
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
  • Shu Huang
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
    • Department of NeurologyShanghai Pudong New Area Gongli Hospital
    • Departments of NeurologyHuashan Hospital, Fudan University
Article

DOI: 10.1007/s11010-011-0806-5

Cite this article as:
Jiang, M., Lv, L., Ji, H. et al. Mol Cell Biochem (2011) 354: 67. doi:10.1007/s11010-011-0806-5

Abstract

Stroke can cause permanent neurological damage, complications, and even death. However, there is no treatment exists to restore its lost function. Human embryonic stems transplantation therapy was a novel and potential therapeutic approach for stroke. However, as we have seen, the ethical controversy pertains to embryonic stem cell research. Human induced pluripotent stem cells (iPSCs) are the latest generation of stem cells that may be a solution to the controversy of using embryonic cells. In our study, we generated iPSCs from adult human fibroblasts by introduction of four defined transcription factors (Oct4, Sox2, Nanog, and Lin-28). And then, we investigated the efficacy of iPSCs transplantation therapy for stroke on the animal models of middle cerebral artery occlusion. Surprisingly, we found that transplanted iPSCs migrated to injured brain areas, and differentiated into neuron-like cells successfully. After 4–16 days iPSCs grafting, sensorimotor function of rats has been improved significantly. In one word, we may prove that iPSCs therapy in stroke to be an effective form of treatment.

Keywords

Brain ischemiaInduced pluripotent stem cellsFibroblastsTranscription factorsTransplantation therapy

Abbreviations

CNS

Central nervous system

ES

Embryonic stem

%HLV

Percentage hemisphere lesion volume

HESC

Human embryonic stem cells

iPSCs

Induced pluripotent stem cells

MCAO

Middle cerebral artery occlusion

SCNT

Somatic cells nuclear transfer

TTC

2,3,5-Triphenyltetrazolium chloride

Copyright information

© Springer Science+Business Media, LLC. 2011