Molecular and Cellular Biochemistry

, Volume 347, Issue 1, pp 175–182

High glucose condition upregulated Txnip expression level in rat mesangial cells through ROS/MEK/MAPK pathway

  • Shaohong Fang
  • Yuhong Jin
  • Haixia Zheng
  • Junxia Yan
  • Yunxia Cui
  • Huimei Bi
  • Huijie Jia
  • Huishu Zhang
  • Yi Wang
  • Liying Na
  • Xu Gao
  • Hongbo Zhou
Article

DOI: 10.1007/s11010-010-0626-z

Cite this article as:
Fang, S., Jin, Y., Zheng, H. et al. Mol Cell Biochem (2011) 347: 175. doi:10.1007/s11010-010-0626-z

Abstract

Thioredoxin interacting protein (Txnip) is one of the most abundantly up-regulated genes in response to hyperglycemia. The increased renal expression of Txnip was associated with type IV collagen accumulation in streptozotocin-induced diabetic mice. As the mechanism of action of high glucose is unknown, we undertook the investigation of the signaling pathway on the upregulation of Txnip expression induced by high glucose in rat mesangial cells. Rat mesangial cells were exposed to normal (5.5 mM) or high (25 mM) glucose at different time points. Txnip expression was determined using real-time RT-PCR and western-blotting at transcription and translation level, respectively. Intracellular reactive oxygen species (ROS) was detected by FACS Calibur flow cytometer using fluorescent probe (DCFH-DA).The treatment with high glucose resulted in an increase of Txnip mRNA from 4 h to 12 h and Txnip protein from 12 to 24 h in comparison with normal glucose condition. In addition, N-acetyl-cysteine (NAC) was found to decrease Txnip protein expression under high glucose condition. Furthermore, p38MAPK inhibitor SB203580 suppressed Txnip expression at transcription and protein level significantly to high glucose exposure. These results suggest that high glucose exposure improves Txnip mRNA and protein expression level by ROS/MEK/MAPK signaling pathway.

Keywords

Thioredoxin-interacting proteinMesangial cellsDiabetic nephropathySignaling pathwayReactive oxygen species

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Shaohong Fang
    • 1
    • 3
  • Yuhong Jin
    • 1
  • Haixia Zheng
    • 1
  • Junxia Yan
    • 1
  • Yunxia Cui
    • 1
  • Huimei Bi
    • 1
  • Huijie Jia
    • 1
  • Huishu Zhang
    • 2
  • Yi Wang
    • 2
  • Liying Na
    • 1
  • Xu Gao
    • 1
  • Hongbo Zhou
    • 1
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyHarbin Medical UniversityHarbinPeople’s Republic of China
  2. 2.Biotechnology Experimental Center for TeachingHarbin Medical UniversityHarbinPeople’s Republic of China
  3. 3.Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and TreatmentHarbin Medical UniversityHarbinPeople’s Republic of China