Molecular and Cellular Biochemistry

, Volume 344, Issue 1, pp 137–150

Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis

  • Xuanwen Li
  • Li Xiong
  • Chunliang Xie
  • Jia Cao
  • Huobao Deng
  • Yong Lin
  • Rui Cao
  • Jianglin Li
  • Ping Chen
  • Songping Liang
Article

DOI: 10.1007/s11010-010-0537-z

Cite this article as:
Li, X., Xiong, L., Xie, C. et al. Mol Cell Biochem (2010) 344: 137. doi:10.1007/s11010-010-0537-z

Abstract

Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during liver regeneration, the PM of liver sinusoidal endothelial cells (LSEC) at 72 h after partial hepatectomy was enriched by an established in vivo membrane density perturbation method. The differentially expressed membrane proteins compared to those from sham operation were quantified using an improved two-dimensional 16-BAC/SDS-PAGE and identified by LC-MS/MS. Several proteins were further confirmed by cICAT labeling quantitative strategy. A total of 47 proteins were identified including known and novel proteins involved in angiogenesis or liver regeneration, such as inducible nitric oxide synthase, type IV collagen, and integrin beta3. Our results indicated that the combination of the membrane density perturbation strategy and the improved two-dimensional electrophoresis (2-DE) method are useful for investigating the endothelial dysfunctions in vivo.

Keywords

16-BAC-PAGEAngiogenesisPartial hepatectomyPlasma membraneProteomics

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Xuanwen Li
    • 1
  • Li Xiong
    • 2
  • Chunliang Xie
    • 1
  • Jia Cao
    • 1
  • Huobao Deng
    • 1
  • Yong Lin
    • 1
  • Rui Cao
    • 1
  • Jianglin Li
    • 1
  • Ping Chen
    • 1
  • Songping Liang
    • 1
  1. 1.Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life SciencesHunan Normal UniversityChangshaPeople’s Republic of China
  2. 2.Division of Hepatobiliary Disease, Department of General Surgerythe Second Xiangya Hospital of Central South UniversityChangshaPeople’s Republic of China