Molecular and Cellular Biochemistry

, Volume 344, Issue 1, pp 1–9

Icariin-mediated differentiation of mouse adipose-derived stem cells into cardiomyocytes

  • Ming-shun Jin
  • Sa Shi
  • Yi Zhang
  • Yan Yan
  • Xiao-dong Sun
  • Wei Liu
  • Hui-wen Liu
Article

DOI: 10.1007/s11010-010-0523-5

Cite this article as:
Jin, M., Shi, S., Zhang, Y. et al. Mol Cell Biochem (2010) 344: 1. doi:10.1007/s11010-010-0523-5
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Abstract

In this study, we investigated the ability of mouse adipose-derived stem cells (ADSCs) to differentiate into a cardiac phenotype in vitro. Icariin (ICA) has previously been shown to induce cardiomyocyte (CM) differentiation of murine embryonic stem cells in vitro, but its effect on ADSCs remains unclear. We isolated ADSCs from white adipose tissue and analyzed selected surface antigens using flow cytometry. ADSCs and CMs were co-cultured in transwell plates, with or without the addition of either ICA or ICA plus the extracellular signal-regulated kinase (ERK) inhibitor PD98059. Cardiac-specific gene expression was examined by reverse transcription-polymerase chain reaction and western blotting. ICA facilitated differentiation of ADSCs into CMs that expressed cardiac-specific genes, including the transcription factors NKX-2.5, GATA-4, MLC-2v, α-actinin, and cardiac troponin-T. Expression of α-actinin, the Z band-constituting protein, was promoted by ICA in a dose- and time-dependent manner. ICA can induce ERK activation and cardiac-specific gene expression was partially inhibited by PD98059 after treatment with ICA. These results suggest that ICA-stimulated CM differentiation of ADSCs, and that it acted partially by activating ERK-dependent signaling pathways in vitro.

Keywords

Adipose-derived stem cell Cardiomyocyte Icariin Differentiation 

Abbreviations

ADSCs

Adipose-derived stem cells

ICA

Icariin

ES

Embryonic stem

cTnT

Cardiac troponin T

CM

Cardiomyocyte

MSCs

Mesenchymal stem cells

PBS

Phosphate-buffered saline

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

FITC

Fluorescein isothiocyanate

DAPI

4′,6′-Diamidino-2-phenylindole dihydrochloride

ERK

Extracellular signal-regulated kinase

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

LV

Left ventricle

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Ming-shun Jin
    • 1
    • 4
  • Sa Shi
    • 2
  • Yi Zhang
    • 1
  • Yan Yan
    • 1
  • Xiao-dong Sun
    • 1
  • Wei Liu
    • 3
  • Hui-wen Liu
    • 1
  1. 1.Department of Histology and EmbryologyHarbin Medical UniversityHarbinChina
  2. 2.Department of PathophysiologyHarbin Medical UniversityHarbinChina
  3. 3.Department of Pathology, The First Affiliated HospitalHarbin Medical UniversityHarbinChina
  4. 4.Department of Anatomy, Histology and EmbryologyHeilongjiang University of Chinese MedicineHarbinChina