Molecular and Cellular Biochemistry

, Volume 341, Issue 1, pp 43–50

Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats

  • Salim M. A. Bastaki
  • Ernest Adeghate
  • Irwin S. Chandranath
  • Naheed Amir
  • Saeed Tariq
  • Rashed S. Hameed
  • Abdu Adem
Article

DOI: 10.1007/s11010-010-0435-4

Cite this article as:
Bastaki, S.M.A., Adeghate, E., Chandranath, I.S. et al. Mol Cell Biochem (2010) 341: 43. doi:10.1007/s11010-010-0435-4

Abstract

Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight). The rats were weighed weekly and sacrificed after 6 months. The glandular portion of the stomach was removed and processed for H+-K+-ATPase immunohistochemistry and light and electron microscopy studies. Acid secretion was measured in vivo. After 6 months of diabetes, the mean weight of the rats was significantly lower (P < 0.001) compared to control. The mean weight of the stomach to body weight percentage increased significantly (P < 0.001) compared to control. The blood glucose level in diabetic rats was significantly higher (P < 0.001) than in normal control. Diabetic rats showed significant (P < 0.001) decrease in basal and stimulated acid secretion when compared to control. Electron micrographs of the parietal cells of glandular stomach of diabetic rats revealed significant (P < 0.0002) reduction in the number of mitochondria and a small though not significant increase in the number of canaliculi in the parietal cells compared with normal. Immunohistochemistry showed reduced H+-K+-ATPase (P < 0.00001) compared to control. Long-term diabetes induces morphological as well as functional changes in gastric parietal cells. The decrease in the number of mitochondria accompanied by reduced in H+-K+-ATPase in parietal cells may explain the reduced acid secretion observed in diabetics.

Keywords

Diabetes mellitusParietal cellsMitochondriaH+, K+-ATPaseAcid secretion

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Salim M. A. Bastaki
    • 1
  • Ernest Adeghate
    • 2
  • Irwin S. Chandranath
    • 1
  • Naheed Amir
    • 1
  • Saeed Tariq
    • 2
  • Rashed S. Hameed
    • 2
  • Abdu Adem
    • 1
  1. 1.Department of Pharmacology, Faculty of Medicine and Health SciencesUAE UniversityAl AinUAE
  2. 2.Department of Anatomy, Faculty of Medicine and Health SciencesUAE UniversityAl AinUAE