Molecular and Cellular Biochemistry

, Volume 338, Issue 1, pp 115–122

High glucose stimulates adipogenic and inhibits osteogenic differentiation in MG-63 cells through cAMP/protein kinase A/extracellular signal-regulated kinase pathway

Authors

  • Weiwei Wang
    • College of Life ScienceGraduate University of Chinese Academy of Sciences
  • Xiaolin Zhang
    • College of Life ScienceGraduate University of Chinese Academy of Sciences
  • Jiaqiang Zheng
    • College of Life ScienceGraduate University of Chinese Academy of Sciences
    • College of Life ScienceGraduate University of Chinese Academy of Sciences
Article

DOI: 10.1007/s11010-009-0344-6

Cite this article as:
Wang, W., Zhang, X., Zheng, J. et al. Mol Cell Biochem (2010) 338: 115. doi:10.1007/s11010-009-0344-6

Abstract

Patients with diabetes tend to have an increased incidence of osteoporosis that may be related to hyperglycemia. In this study, we investigated the effects of high glucose on differentiation of human osteoblastic MG-63 cells and involved intracellular signal transduction pathways. Here, we showed that high glucose suppressed the cell growth, mineralization, and expression of osteogenic markers including Runx2, collagen I, osteocalcin, osteonectin, but inversely promoted expression of adipogenic markers including PPARγ, aP2, resistin, and adipsin. Moreover, high glucose significantly increased the intracellular cAMP level in a time-dependent manner and induced ERK1/2 activation. Meanwhile, supplementation of H89, a specific inhibitor of PKA, and PD98059, a specific inhibitor of MAPK/ERK kinase, reversed the cell growth inhibition, the down-regulation of osteogenic markers and the up-regulation of adipogenic markers as well as the activation of ERK under high glucose. These results indicate that high glucose can increase adipogenic and inhibit osteogenic differentiation by activating cAMP/PKA/ERK pathway in MG-63 cells, thereby providing further insight into the molecular mechanism of diabetic osteoporosis.

Keywords

High glucose Osteogenic differentiation Adipogenic differentiation cAMP Protein kinase A Extracellular signal-regulated kinase

Copyright information

© Springer Science+Business Media, LLC. 2009