Molecular and Cellular Biochemistry

, Volume 335, Issue 1, pp 283–289

Retinoid X receptor agonists inhibit phorbol-12-myristate-13-acetate (PMA)-induced differentiation of monocytic THP-1 cells into macrophages

  • Lei Zhou
  • Ling-hong Shen
  • Liu-hua Hu
  • Heng Ge
  • Jun Pu
  • Da-jun Chai
  • Qin Shao
  • Li Wang
  • Jin-zhang Zeng
  • Ben He
Article

DOI: 10.1007/s11010-009-0278-z

Cite this article as:
Zhou, L., Shen, L., Hu, L. et al. Mol Cell Biochem (2010) 335: 283. doi:10.1007/s11010-009-0278-z

Abstract

Monocyte/macrophage differentiation is an essential process during atherosclerosis development. The retinoid X receptor (RXR) is a member of the nuclear hormone receptor superfamily, which plays an important regulatory role in many metabolic disorders, including atherosclerosis. The purpose of this study was to investigate the effect of RXR agonist on monocyte/macrophage differentiation in vitro. The THP-1 cell line was differentiated into a macrophage-like phenotype by incubation with phorbol-12-myristate-13-acetate (PMA) in the presence or absence of RXR agonist. The viability of adherent differentiated THP-1 cells was determined by MTT assay. Macrophage surface marker CD11b and CD36 was analyzed by flow cytometry. Phagocytosis was measured by fluorescence-labeled latex beads. The production of Cytokine Tunlornecrosisfactor-α (TNF-α), Interlaken-12p70 (IL-12p70), and Matrix metalloproteinase-9 (MMP-9), each of which was analyzed by ELISA. In the presence of the RXR agonists 9-cis retinoic acid or SR11237, PMA-induced THP-1 cells became less adherent, showed decreased macrophage-like morphological changes, decreased cell surface antigen CD11b and CD36 expression, and down regulated the phagocytosis of latex beads and the production of TNF-α and MMP-9. These data suggest that RXR agonists inhibit PMA-induced THP-1 cell differentiation into macrophage-like cells, which may be helpful in understanding the anti-atherosclerotic effect of RXR and its agonists.

Keywords

Retinoid X receptorMonocyteMacrophageDifferentiation

Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Lei Zhou
    • 1
  • Ling-hong Shen
    • 1
  • Liu-hua Hu
    • 1
  • Heng Ge
    • 1
  • Jun Pu
    • 1
  • Da-jun Chai
    • 2
  • Qin Shao
    • 1
  • Li Wang
    • 3
  • Jin-zhang Zeng
    • 3
    • 4
  • Ben He
    • 1
  1. 1.Department of Cardiology, Renji HospitalMedical College of Shanghai Jiaotong UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Cardiologythe first affiliated hospital of Fujian Medical UniversityFuzhouPeople’s Republic of China
  3. 3.Shanghai Institutes for Biological ScienceChinese Academy of SciencesShanghaiPeople’s Republic of China
  4. 4.Institute for Biomedical ResearchXiamen UniversityXiamenChina