Molecular and Cellular Biochemistry

, Volume 324, Issue 1, pp 85–91

Differential activation of mitogen-activated protein kinases following high and low LET radiation in murine macrophage cell line

  • Himanshi Narang
  • Nagesh Bhat
  • S. K. Gupta
  • S. Santra
  • R. K. Choudhary
  • S. Kailash
  • Malini Krishna
Article

DOI: 10.1007/s11010-008-9987-y

Cite this article as:
Narang, H., Bhat, N., Gupta, S.K. et al. Mol Cell Biochem (2009) 324: 85. doi:10.1007/s11010-008-9987-y

Abstract

Mitogen-activated protein kinases have been shown to respond to various stimuli including cytokines, mitogens and gamma irradiation, leading to cell proliferation, differentiation, or death. The duration of their activation determines the specificity of response to each stimulus in various cells. In this study, the crucial intracellular kinases, ERK, JNK, and p38 kinase involved in cell survival, death, or damage and repair were examined for their activity in RAW 264.7 cells at various time points after irradiation with 2 Gy doses of proton ions or X-rays. This is the first report that shows that the MAPK signaling induced after heavy ion or X-ray exposure is not the same. Unlike gamma irradiation, there was prolonged but marginal activation of prosurvival ERK pathway and significant activation of proapoptotic p38 pathway in response to high LET radiation.

Keywords

Mitogen Activated Protein KinasesERKJNKp38GammaHigh LET radiation

Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Himanshi Narang
    • 1
  • Nagesh Bhat
    • 2
  • S. K. Gupta
    • 3
  • S. Santra
    • 3
  • R. K. Choudhary
    • 3
  • S. Kailash
    • 3
  • Malini Krishna
    • 1
  1. 1.Radiation Biology and Health Sciences DivisionBhabha Atomic Research CentreTrombay, MumbaiIndia
  2. 2.Radiological Protection and Dosimetry DivisionBhabha Atomic Research CentreTrombay, MumbaiIndia
  3. 3.Nuclear Physics DivisionBhabha Atomic Research CentreTrombay, MumbaiIndia