Article

Molecular and Cellular Biochemistry

, Volume 317, Issue 1, pp 61-68

hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence

  • Yu-Bo ZhouAffiliated withChinese National Human Genome Center at Shanghai
  • , Jia-Bing CaoAffiliated withChinese National Human Genome Center at Shanghai
  • , Bing-Bing WanAffiliated withChinese National Human Genome Center at Shanghai
  • , Xin-Rong WangAffiliated withChinese National Human Genome Center at Shanghai
  • , Guo-Hui DingAffiliated withBioinformatics Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
  • , Hong ZhuAffiliated withChinese National Human Genome Center at Shanghai
  • , Hong-Meng YangAffiliated withChinese National Human Genome Center at Shanghai
  • , Ke-Sheng WangAffiliated withChinese National Human Genome Center at Shanghai
  • , Xin ZhangAffiliated withChinese National Human Genome Center at Shanghai

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Abstract

This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Furthermore, the secretion was not dependent on its predicted signal peptide. All the experiment results suggested that the secretion was a non-classical export. Phylogenetic analysis showed that the human BolAs belong to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among hBolA1, hBolA2, and hBolA3 could be responsible for their functional divergence. Our data provided a basis for functional studies of BolA protein family.

Keywords

hBolA1 hBolA2 hBolA3 Non-classic secretion BolA protein family Functional divergence