Identification and molecular characterization of two hepcidin genes from black rockfish (Sebastes schlegelii)

  • Young-Ok Kim
  • Eun-Mi Park
  • Bo-Hye Nam
  • Hee Jeong Kong
  • Woo-Jin Kim
  • Sang-Jun Lee
Article

DOI: 10.1007/s11010-008-9796-3

Cite this article as:
Kim, YO., Park, EM., Nam, BH. et al. Mol Cell Biochem (2008) 315: 131. doi:10.1007/s11010-008-9796-3

Abstract

Antimicrobial peptides (AMPs) are important mediators of the immune response against bacteria and cysteine-rich peptide hepcidin is a 20–26 residues member with functions in iron regulation and antimicrobial activity. Here, we have identified two different types of hepcidin cDNA from the Black rockfish, Sebastes schlegelii, by EST analysis. Both hepcidin genes (hepcidin I and II) consist of two introns and three exons that encode a prepropeptide (88 amino acids). A TATA box and several consensus-binding motifs for transcription factors were found in the upstream of the transcriptional start site. Semi-quantitative RT-PCR analysis suggested that hepcidin I transcripts were detected in various tissues, while hepcidin II was only expressed in the liver. During the bacterial challenge with the fish pathogen, Streptococcus iniae, two hepcidin genes were differentially expressed. Hepcidin I and II dramatically increased at 24 h post-injection, then gradually declined at 3 days in hepcidin II, while hepcidin I expression continued at 3 days after challenge.

Keywords

Hepcidin Antimicrobial peptide Sebastes schlegelii Gene expression 

Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Young-Ok Kim
    • 1
  • Eun-Mi Park
    • 1
  • Bo-Hye Nam
    • 1
  • Hee Jeong Kong
    • 1
  • Woo-Jin Kim
    • 1
  • Sang-Jun Lee
    • 1
  1. 1.National Fisheries Research and Development InstituteBiotechnology Research InstituteBusanRepublic of Korea