Molecular and Cellular Biochemistry

, Volume 310, Issue 1, pp 227–234

External Qi of Yan Xin Qigong induces G2/M arrest and apoptosis of androgen-independent prostate cancer cells by inhibiting Akt and NF-κB pathways

Authors

  • Xin Yan
    • Institute of Chongqing Traditional Chinese Medicine
    • New Medical Science Research Institute
  • Hua Shen
    • New Medical Science Research Institute
  • Hongjian Jiang
    • Brigham and Women’s HospitalHarvard Medical School
  • Chengsheng Zhang
    • McMaster University
  • Dan Hu
    • Dana-Farber Cancer InstituteHarvard Medical School
  • Jun Wang
    • New Medical Science Research Institute
    • Brigham and Women’s HospitalHarvard Medical School
Article

DOI: 10.1007/s11010-007-9684-2

Cite this article as:
Yan, X., Shen, H., Jiang, H. et al. Mol Cell Biochem (2008) 310: 227. doi:10.1007/s11010-007-9684-2

Abstract

Long-term clinical observations and ongoing studies have shown antitumor effects of external Qi of Yan Xin Qigong (YXQG-EQ) that originated from traditional Chinese medicine (TCM). In order to understand the molecular mechanisms underlying the antitumor effects of YXQG-EQ, we investigate the effects of YXQG-EQ on growth and apoptosis in androgen-independent prostate cancer PC3 cells. We found that exposure to YXQG-EQ led to G2/M arrest associated with reduced cyclin B1 expression and apoptosis in PC3 cells. YXQG-EQ treatment inhibited constitutive and epidermal growth factor-induced Akt phosphorylation, basal and TNF-α-induced NF-κB activation, and downregulated anti-apoptotic Bcl-2 and Bcl-xL expression. In contrast, exposure to YXQG-EQ increased phosphorylation of Akt and Erk1/2 in human umbilical vein endothelial cells (HUVEC), and had no cytotoxic effect on either HUVEC or peripheral blood mononuclear cells (PBMC). These results indicate that YXQG-EQ has profound effects on growth and apoptosis of prostate cancer cells by targeting survival pathways including the Akt and NF-κB pathways.

Keywords

AktNF-κBProstate cancerG2/M arrestApoptosisExternal Qi of Yan Xin Qigong

Copyright information

© Springer Science+Business Media, LLC. 2007