Molecular and Cellular Biochemistry

, Volume 304, Issue 1, pp 297–304

Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2

  • Li Li
  • Yan Shi
  • Huiling Wu
  • Bingbing Wan
  • Pu Li
  • Li Zhou
  • Huili Shi
  • Keke Huo
Article

DOI: 10.1007/s11010-007-9512-8

Cite this article as:
Li, L., Shi, Y., Wu, H. et al. Mol Cell Biochem (2007) 304: 297. doi:10.1007/s11010-007-9512-8

Abstract

HCCA2 (hepatocellular carcinoma-associated gene 2) was initially identified as a HCC (hepatocellular carcinoma)-specific protein and subsequently, a long splice variant of HCCA2 was identified as a co-activator of transcription factor YY1 (Yin Yang 1). To investigate the role of HCCA2 in HCC genesis and progression, we screened a human fetal liver cDNA library and identified a novel HCCA2-interacting protein, MAD2L2 (MAD2 mitotic arrest deficient-like 2 (yeast)). The interaction between HCCA2 and MAD2L2 was confirmed by in vitro and in vivo binding assays and the interaction domain was mapped to the N-terminus of HCCA2 by sequential deletion. HCCA2 and MAD2L2 also colocalized in the nucleus of Hela cells. Furthermore, overexpression of HCCA2 led to cell cycle arrest at G0/G1 phase and therefore inhibited cell proliferation. Our research suggests that HCCA2 may play a novel role in cell cycle regulation.

Keywords

HCCA2MAD2L2Protein–protein interactionCell cycle

Supplementary material

11010_2007_9512_MOESM1_ESM.pdf (180 kb)
(PDF 14 kb)

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Li Li
    • 1
  • Yan Shi
    • 1
  • Huiling Wu
    • 1
  • Bingbing Wan
    • 1
  • Pu Li
    • 1
  • Li Zhou
    • 1
  • Huili Shi
    • 1
  • Keke Huo
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan UniversityShanghaiChina