Molecular and Cellular Biochemistry

, Volume 304, Issue 1, pp 71–77

Involvement of PPAR-γ and p53 in DHA-induced apoptosis in Reh cells

  • Hamid Zand
  • Ali Rhimipour
  • Masoomeh Bakhshayesh
  • Mohammad Shafiee
  • Issa Nour Mohammadi
  • Saideh Salimi
Article

DOI: 10.1007/s11010-007-9487-5

Cite this article as:
Zand, H., Rhimipour, A., Bakhshayesh, M. et al. Mol Cell Biochem (2007) 304: 71. doi:10.1007/s11010-007-9487-5

Abstract

Docosahexaenoeic acid (DHA, 22:6 n-3) is an omega-3 polyunsaturated fatty acid that is found in fish oil and exerts cytotoxic effect on a variety of cell lines. The molecular target, responsible for mediating this effect of DHA, still remains unknown. In this report, we presented experimental evidences for the role of PPAR-γ in conveying the cytotoxic effect of DHA. We showed that DHA induces apoptosis in Reh and Ramos cells and apoptotic effect of DHA is inhibited by the PPAR-γ antagonist GW9662, indicating that PPAR-γ functions as the mediator of the apoptotic effect of DHA. Furthermore, our result showed that DHA induces the PPAR-γ protein levels in both Reh and Ramos cells. Interestingly, DHA was found to induce the expression of p53 protein in Reh cells in a PPAR-γ-dependent manner. The up-regulation of p53 protein by DHA kinetically correlated with the activation of caspase 9, caspase 3, and induction of apoptosis, suggesting a role for p53 in DHA-mediated apoptosis in Reh cells. Taken together, these findings suggest a new signaling pathway, DHA-PPAR-γ-p53, in mediating the apoptotic effect of DHA in Reh cells.

Keywords

DHAPolyunsaturated fatty acidsPPAR- γApoptosisp53

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hamid Zand
    • 1
  • Ali Rhimipour
    • 1
  • Masoomeh Bakhshayesh
    • 2
  • Mohammad Shafiee
    • 2
  • Issa Nour Mohammadi
    • 2
  • Saideh Salimi
    • 2
  1. 1.Faculty of Medicine, Department of BiochemistryTabriz Medical Sciences UniversityTabrizIran
  2. 2.Cellular and Molecular Biology Research CenterIran University Medical SciencesTehranIran