Molecular and Cellular Biochemistry

, Volume 297, Issue 1, pp 131–138

Caffeic acid phenethyl ester suppresses oxidative stress in Escherichia coli-induced pyelonephritis in rats


    • Department of Biochemistry, Faculty of Veterinary MedicineMustafa Kemal University
  • Sadik Gorur
    • Department of Urology, Medicine FacultyMustafa Kemal University
  • Ozkan Aslantas
    • Department of Microbiology, Faculty of Veterinary MedicineMustafa Kemal University
  • Suat Erdogan
    • Department of Biochemistry, Faculty of Veterinary MedicineMustafa Kemal University
  • Sabahattin Ocak
    • Department of Infectious Diseases and Clinical Microbiology, Medicine FacultyMustafa Kemal University
  • Sibel Hakverdi
    • Department of Pathology, Medicine FacultyMustafa Kemal University

DOI: 10.1007/s11010-006-9337-x

Cite this article as:
Celik, S., Gorur, S., Aslantas, O. et al. Mol Cell Biochem (2007) 297: 131. doi:10.1007/s11010-006-9337-x


Although oxidative damage is known to be involved in inflammatory-mediated tissue destruction, modulation of oxygen free radical production represents a new approach to the treatment of inflammatory diseases. Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, has antioxidant, anti-inflammatory and antibacterial properties. For that reason, we aimed to investigate the efficiency of CAPE administration in preventing oxidative damage in pyelonephritis (PYN) caused by Escherichia coli. In this study, 35 Wistar rats were grouped as follows: control, PYN 24 h, PYN 48 h, PYN 72 h, CAPE 24 h, CAPE 48 h and CAPE 72 h. E. coli (1 × 109 c.f.u.) were inoculated into the rats in both PYN and CAPE groups via urethral catheterization. Ten μM/kg-body weight CAPE was injected to the rats in all CAPE groups 24 h before E. coli infection, and injections were repeated at 24-h intervals. Rats were sacrificed 24 h, 48 h and 72 h after infection in both PYN and CAPE groups. Malondialdehyde (MDA) and nitric oxide (NO) levels were significantly increased in kidneys of PYN groups. The activities of the antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and xanthine oxidase (XO) were also elevated by E. coli. However, CAPE administration reduced MDA and NO levels, as well as XO activity, although it increased SOD and GSH-Px activities. Histopathological examination showed that CAPE reduced the inflammation grade induced by E. coli. In conclusion, CAPE administrations decrease the oxidative damage occurring in PYN and therefore could be used for medical management of bacterial nephropathy.


caffeic acid phenethyl esteroxidative damagepreventionpyelonephritis

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© Springer Science+Business Media, LLC 2006