Molecular and Cellular Biochemistry

, Volume 295, Issue 1, pp 167–177

Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-β1

  • Wei Guo
  • Hong Xu
  • Jing Chen
  • Yong Yang
  • Jia Wei Jin
  • Rui Fu
  • Hai Mei Liu
  • Xi Liang Zha
  • Zhi Gang Zhang
  • Wen Yan Huang
Article

DOI: 10.1007/s11010-006-9286-4

Cite this article as:
Guo, W., Xu, H., Chen, J. et al. Mol Cell Biochem (2007) 295: 167. doi:10.1007/s11010-006-9286-4

Abstract

Prohibitin (PHB), a potential tumor suppressor, has been shown to inhibit cell proliferation by repressing E2F-mediated transcription. But little is known about the role of PHB involved in tubulointerstitial fibrosis (TIF). Here, for the first time, we found PHB protein was positively expressed at normal renal tissues, strongly down-regulated in renal biopsy specimens, and negatively correlated with the expression of alpha-smooth-muscle actin (α-SMA) and with the degrees of tubulointerstitial lesions. Transforming growth factor-β1 (TGF-β1) is the most important profibrotic cytokine in the process of TIF and capable of inducing cell phenotypic change of interstitial fibroblasts characterized by the de novo expression of α-SMA. Confocal microscopy showed majority of PHB is located at cytoplasm as well as at nucleus in rat kidney fibroblasts cell (NRK-49F). As we found that PHB protein and mRNA expression were down-regulated in NRK-49F cells following TGF-β1 stimulation. We used transient transfection to over-express PHB protein and found that cells with increased PHB levels had a significant reduction in the percentage entering cell cycle and abolished de novo expression of α-SMA following TGF-β1 stimulation. Therefore, over-expression of PHB suppresses renal interstitial fibroblasts proliferation and cell phenotypic change induced by TGF-β1, which indicates PHB as a potential therapeutic target to halt the progression of TIF.

Keywords

prohibitinfibroblasttransforming growth factor-β1cell cyclecell phenotypic changetubulointerstitial lesions

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Wei Guo
    • 1
  • Hong Xu
    • 1
  • Jing Chen
    • 1
  • Yong Yang
    • 2
  • Jia Wei Jin
    • 2
  • Rui Fu
    • 1
  • Hai Mei Liu
    • 1
  • Xi Liang Zha
    • 2
  • Zhi Gang Zhang
    • 3
  • Wen Yan Huang
    • 1
  1. 1.Department of NephrologyChildren’s Hospital of Fudan UniversityShanghaiP.R. China
  2. 2.Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical CollegeFudan UniversityShanghaiP.R. China
  3. 3.Department of Pathology, Shanghai Medical CollegeFudan UniversityShanghaiP.R. China