Molecular and Cellular Biochemistry

, Volume 289, Issue 1, pp 185–191

(–)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes

Authors

  • Po Yee Chiu
    • Department of BiochemistryThe Hong Kong University of Science & Technology
  • Hoi Yan Leung
    • Department of BiochemistryThe Hong Kong University of Science & Technology
  • Michel K. T. Poon
    • Department of BiochemistryThe Hong Kong University of Science & Technology
  • Duncan H. F. Mak
    • Department of BiochemistryThe Hong Kong University of Science & Technology
    • Section of Molecular Hematology and Therapy, Department of Blood and Marrow TransplantationUT MD Anderson Cancer Center
    • Department of BiochemistryThe Hong Kong University of Science & Technology
Article

DOI: 10.1007/s11010-006-9163-1

Cite this article as:
Chiu, P.Y., Leung, H.Y., Poon, M.K.T. et al. Mol Cell Biochem (2006) 289: 185. doi:10.1007/s11010-006-9163-1

Abstract

Effects of schisandrin B enantiomers ((+)Sch B and (–)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (–)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (–)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (–)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (–)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.

Keywords

schisandrin B enantiomers glutathione heat shock protein H9c2 cardiomyocytes

Abbreviation

 

FBS

fetal bovine serum

 

GSH

reduced glutathione

 

Hsp

heat shock protein

 

LDH

lactate dehydrogenase

 

PBS

phosphate-buffered saline

 

Sch B

schisandrin B

 

XO

Xanthine oxidase

Copyright information

© Springer Science+Business Media, Inc. 2006