Molecular and Cellular Biochemistry

, Volume 286, Issue 1, pp 161–170

Identification and characterization of recombinant and native rat myristoyl-CoA: protein N-myristoyltransferases

Authors

  • Vincent Rioux
    • Laboratoire de BiochimieINRA-Agrocampus
  • Erwan Beauchamp
    • Laboratoire de BiochimieINRA-Agrocampus
  • Frédérique Pedrono
    • Laboratoire de BiochimieINRA-Agrocampus
  • Stéphanie Daval
    • Laboratoire de BiochimieINRA-Agrocampus
  • Daniel Molle
    • UMR STLOINRA-Agrocampus
  • Daniel Catheline
    • Laboratoire de BiochimieINRA-Agrocampus
    • Laboratoire de BiochimieINRA-Agrocampus
    • Laboratoire de BiochimieINRA-Agrocampus
Article

DOI: 10.1007/s11010-005-9108-0

Cite this article as:
Rioux, V., Beauchamp, E., Pedrono, F. et al. Mol Cell Biochem (2006) 286: 161. doi:10.1007/s11010-005-9108-0

Abstract

Compared to other species that possess a single functional myristoyl-CoA: protein N-myristoyltransferase gene copy, human, mouse and cow possess 2 NMT genes, and more than 2 protein isoforms. In mammals, the contribution of each gene transcript to multiple protein isoform expression and enzyme activity remains unclear. In order to get new insight on their respective physiological role, we have cloned and characterized the two rat NMT cDNAs. Rat NMT1 and NMT2 cDNAs contain 1491 and 1590 nucleotides, respectively, with high identity with their mouse homologues. Polypeptide sequences exhibited 68.1% identity between NMT1 and 2. Recombinant rat NMT1 and 2 showed major immunoreactive forms at 66 and 50 kDa, although NMT2 is 33-amino acid longer than NMT1. Both proteins exhibited functional myristoyltransferase activity but NMT2 appeared to be 4-time less active than NMT1. Studies of native protein expression revealed that the level and sizes of NMT proteins greatly vary among rat tissues although NMT1 and 2 did not display tissue specific expression at the mRNA level. Altogether, these results suggest that NMT2 may contribute little to total NMT activity levels in vivo.

Keywords

NMTN-terminal myristoylationmyristic acidsaturated fatty acid metabolismrat

Abbreviations:

NMT

myristoyl-CoA: protein N-myristoyltransferase

hNMT

human NMT

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Copyright information

© Springer Science+Business Media, Inc. 2005