Molecular and Cellular Biochemistry

, Volume 277, Issue 1, pp 117–125

High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2)

Authors

  • Yunfeng Li
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
    • Department of Biological ChemistryUniversity of California
  • Yongsheng Chang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Lifeng Zhang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Qiping Feng
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Zhuo Liu
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Yongwei Zhang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Jin Zuo
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Yan Meng
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
  • Fude Fang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences
Article

DOI: 10.1007/s11010-005-5535-1

Cite this article as:
Li, Y., Chang, Y., Zhang, L. et al. Mol Cell Biochem (2005) 277: 117. doi:10.1007/s11010-005-5535-1

Abstract

A new Rattus norvegicus PanK gene was isolated by mRNA differential display from high concentration glucose-stimulated rat, which encodes a human PanK4-like protein with KOG2201 and KOG4584 domain. Proteins that interact with rat PanK4 were identified by the application of the yeast two-hybrid system. One of the components, Pkm2, was found to be associated with rat PanK4 and its two domains under both in vitro and in vivo conditions. Immunofluorescence staining and confocal scanning experiments showed that PanK4 could transiently co-express with Pkm2 in the cytoplasm of HeLa cell and HEK293T cell. These findings suggest that PanK4 interacts with Pkm2 and thereby may modulate the glucose metabolism through regulating the activity of Pkm2.

Keywords

blood glucose regulationyeast two-hybrid systemglucose metabolismtype 2 diabetes

Abbreviation:

PanK

Pantothenate kinase

Pkm2

M2-type pyruvate kinase

aa

amino acid residues

GST

glutathione-S-transferase

PBS

phosphate-buffered saline

HEK

human embryonic kidney

MoAb

monoclonal antibody

Copyright information

© Springer Science + Business Media, Inc. 2005