Molecular and Cellular Biochemistry

, Volume 269, Issue 1, pp 85–94

Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells

  • Hongying Sun
  • Nan Li
  • Xiaojian Wang
  • Taoyong Chen
  • Liyun Shi
  • Lihuang Zhang
  • Jianli Wang
  • Tao Wan
  • Xuetao Cao
Article

DOI: 10.1007/s11010-005-2539-9

Cite this article as:
Sun, H., Li, N., Wang, X. et al. Mol Cell Biochem (2005) 269: 85. doi:10.1007/s11010-005-2539-9

Abstract

Vertebrates have muscle and non-muscle isozymes of adenylosuccinate synthetase (AdSS, EC 6.3.4.4), which catalyzes the first committed step in AMP synthesis. A novel muscle isozyme of adenylosuccinate synthetase, human AdSSL1, is identified from human bone marrow stromal cells. AdSSL1 is 98% identical to mouse muscle type AdSS1 and contains conserved sequence and structural features of adenylosuccinate synthetase. Human AdSSL1 gene is mapped to chromosome 14p32.33. After stimulation, leukemia cells express AdSSL1 in a time-dependent manner different from that of non-muscle adenylosuccinate synthetase. The human AdSSL1 is predominantly expressed in skeletal muscle and cardiac tissue consistent with the potential role for the enzyme in muscle metabolism. Overexpressed AdSSL1 protein in COS-7 cells locates in cytoplasm. Recombinant AdSSL1 protein possesses typical enzymatic activity to catalyze adenylosuccinate formation. The identification of human AdSSL1 with predominant expression in muscle tissue will facilitate future genetic and biochemical analysis of the enzyme in muscle physiology. (Mol Cell Biochem 269: 85–94, 2005)

Keywords

bone marrow stromal celladenylosuccinate synthetasepurine nucleotide metabolismgene cloning

Abbreviation

AdSS

adenylosuccinate synthetase;

AdSSL1

adenylosuccinate synthetase-like 1;

BMSC

bone marrow stromal cell;

FITC

fluorescein isothiocyanate;

ORF

open reading frame;

LPS

lipopolysaccharide;

GST

Glutathione S-transferase;

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Hongying Sun
    • 1
  • Nan Li
    • 2
  • Xiaojian Wang
    • 1
  • Taoyong Chen
    • 2
  • Liyun Shi
    • 1
  • Lihuang Zhang
    • 1
  • Jianli Wang
    • 2
  • Tao Wan
    • 1
  • Xuetao Cao
    • 1
  1. 1.Institute of ImmunologySecond Military Medical UniversityShanghaiP.R. China
  2. 2.Institute of ImmunologyZhejiang UniversityHangzhouP.R. China.