Article

International Journal of Peptide Research and Therapeutics

, 17:225

First online:

Effect of a Pool of Peptides Isolated from Crotalus durissus terrificus (South American Rattlesnake) Venom on Glucose Levels of Mice Fed on a High-Fat Diet

  • M. E. S. Martins-SantosAffiliated withMidwest Campus (CCO), Federal University of São João Del Rei (FUSJ)
  • , R. R. ResendeAffiliated withMidwest Campus (CCO), Federal University of São João Del Rei (FUSJ)
  • , F. C. H. PintoAffiliated withMidwest Campus (CCO), Federal University of São João Del Rei (FUSJ)
  • , A. M. SoaresAffiliated withDepartment of Clinical Analysis of the Faculty of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University
  • , S. MarangoniAffiliated withDepartment of Biochemistry of Biology Institute, State University of Campinas
  • , E. OliveiraAffiliated withProteomics Platform, Barcelona Science Park, University of Barcelona
  • , F. AlbericioAffiliated withInstitute for Research in Biomedicine, Barcelona Science Park, University of BarcelonaCIBER-BBN, Networking Centre on Bioengineering Biomaterials and Nanomedicine, Barcelona Science ParkDepartment of Organic Chemistry, University of Barcelona
  • , S. L. Da SilvaAffiliated withMidwest Campus (CCO), Federal University of São João Del Rei (FUSJ) Email author 

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Abstract

This study investigated the effect of a pool of peptides, isolated from venom of Crotalus durissus terrificus (South American rattlesnake) on glucose concentration in C57BL/6 mice fed on a high-fat diet for 6 weeks. The pool of peptides (molecular mass around of 10 kDa) was obtained using a MidJet apparatus with a cartridge of 10 KDa. The peptide pool was injected intraperitoneally in mice in a single dose (0.5 mg/animal) or multiple doses (0.2 mg/dose). After predetermined times (30, 60, 90 and 120 min) post injections, venous blood samples were collected for enzymatic measurement of serum glucose using a commercial glucose kit (glucose oxidase method). High-fat fed mice showed an increase in blood glucose concentration, in comparison with mice fed on the chow diet. Thirty minutes after a single dose of the peptide pool, high-fat fed animals showed a significant decrease (~47%) in glycemia. However, the glucose level increased again at 60 and 120 min. Conversely, after multiple injections of the pool of peptides administered every 30 min, the blood glucose concentration in the high-fat mice was significantly decreased (~37%) and remained at low levels until 120 min. These results suggest that the tested pool of peptides from Crotalus durissus terrificus contained a peptide (or peptides) with a beneficial role on glucose-lowering action of high-fat fed mice.

Keywords

Bioactive peptides Crotalus durissus terrificus Snake venom Hyperglycemia Glucose