Isolation and Characterization of a Natriuretic Peptide from Crotalus oreganus abyssus (Grand Canyon Rattlesnake) and its Effects on Systemic Blood Pressure and Nitrite Levels

  • S. L. Da Silva
  • J. R. Almeida
  • L. M. Resende
  • W. Martins
  • F. A. F. A. Henriques
  • P. A. Baldasso
  • A. M. Soares
  • A. G. Taranto
  • R. R. Resende
  • S. Marangoni
  • C. A. Dias-Junior
Article

DOI: 10.1007/s10989-011-9254-z

Cite this article as:
Da Silva, S.L., Almeida, J.R., Resende, L.M. et al. Int J Pept Res Ther (2011) 17: 165. doi:10.1007/s10989-011-9254-z

Abstract

Studies on the therapeutic potential of venom peptides have significantly advanced the development of new peptide drugs. A good example is captopril, a synthetic peptide drug, which acts as an anti-hypertensive and potentiating bradykinin, inhibiting the angiotensin-converting enzyme, whose precursor was isolated from the venom of Bothrops jararacussu. The natriuretic peptide (NPs) family comprises three members, ANP (atrial natriuretic peptide), BNP (B-type natriuretic peptide) and CNP (C-type natriuretic peptide), and has an important role in blood pressure regulation and electrolyte homeostasis. In this study, we describe, for the first time, the isolation and characterization of a novel natriuretic-like peptide (Coa_NP), isolated from Crotalus Oreganus abyssus venom. The peptide has 32 amino acids and its complete sequence is SKRLSNGCFGLKLDRIGAMSGLGCWRLINESK. The Coa_NP has an average molecular mass of 3510.98 Da and its amino acid sequence presents the loop region that is characteristic of natriuretic peptides (17 amino acids, NP domain consensus; CFGXXXDRIXXXSGLGC). Coa_NP is a natriuretic peptide of the ANP/BNP-like family, since the carboxy terminal region of CNP has its own NP domain. The functional experiments showed that Coa_NP produced biological effects similar to those of the other natriuretic peptides: (1) a dose-dependent decrease in mean arterial pressure; (2) significant increases in plasma nitrite levels, and (3) vasorelaxation in thoracic aortic rings that were pre-contracted with phenylephrine. The structural and biological aspects confirm Coa_NP as a natriuretic peptide isolated from snake venom, thus expanding the diversification of venom components.

Keywords

Natriuretic peptide Crotalus oreganus abyssus Bioactive peptides Nitric oxide 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • S. L. Da Silva
    • 1
  • J. R. Almeida
    • 1
  • L. M. Resende
    • 1
  • W. Martins
    • 1
  • F. A. F. A. Henriques
    • 1
  • P. A. Baldasso
    • 2
  • A. M. Soares
    • 3
  • A. G. Taranto
    • 1
  • R. R. Resende
    • 1
  • S. Marangoni
    • 2
  • C. A. Dias-Junior
    • 1
  1. 1.Federal University of São João Del Rei, Campus Centro-oesteDivinopolisBrazil
  2. 2.Department of BiochemistryInstitute of Biology, State University of CampinasCampinasBrazil
  3. 3.Department of ToxicologyFaculty of Phamaceutics Sciences, São Paulo UniversityRibeirão PretoBrazil

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