International Journal of Peptide Research and Therapeutics

, Volume 12, Issue 4, pp 373–377

Antiviral Activity of Antimicrobial Lipopeptide from Bacillus subtilis fmbj Against Pseudorabies Virus, Porcine Parvovirus, Newcastle Disease Virus and Infectious Bursal Disease Virus in Vitro

  • Xianqing Huang
  • Zhaoxin Lu
  • Haizhen Zhao
  • Xiaomei Bie
  • FengXia Lü
  • Shujing Yang
Article

DOI: 10.1007/s10989-006-9041-4

Cite this article as:
Huang, X., Lu, Z., Zhao, H. et al. Int J Pept Res Ther (2006) 12: 373. doi:10.1007/s10989-006-9041-4

Bacillus subtilis fmbj can produce lipopeptide antimicrobial substance, whose main components were surfactin and fengycin. In the study, the antiviral activity of antimicrobial lipopeptides (AMLs) from B. subtilis fmbj (CGMCC No. 0934) against Pseudorabies Virus (PRV), Porcine Parvovirus (PPV), Newcastle Disease Virus (NDV) and Infectious Bursal Disease Virus (IBDV) was evaluated in vitro. The AMLs represented a direct inactivation effect on cell-free virus stocks of PRV, PPV, NDV and IBDV, and it could effectively inhibit infection and replication of the NDV and IBDV, but failed to affect PRV and PPV. The AMLs were represented higher toxicity for the Porcine Kidney (PK-15) cells (50% cytotoxic concentration (CC50) value was 32.87 μM) and lower for the Chicken Embryo Fibroblasts (CEF) cells (CC50 value was 89.16 μM). The Selectivity index of AMLs on PRV, PPV, NDV and IBDV was 1.44, 2.23, 8.40 and 12.19, respectively.

Keywords

AMLsBacillus sp. fmbjInfectious Bursal Disease VirusNewcastle Disease VirusPorcine ParvovirusPseudorabies Virus

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Xianqing Huang
    • 1
  • Zhaoxin Lu
    • 1
  • Haizhen Zhao
    • 1
  • Xiaomei Bie
    • 1
  • FengXia Lü
    • 1
  • Shujing Yang
    • 2
  1. 1.College of Food Science and TechnologyNanjing Agricultural UniversityNanjingP.R. China
  2. 2.Bioengineering of Shandong Boly-lely Co. Ltd.Tai’anP.R. China
  3. 3.College of Food Science and TechnologyNanjing Agricultural UniversityNanjingP.R. China