Journal of Muscle Research and Cell Motility

, Volume 30, Issue 1, pp 67–72

Raf-1: a novel cardiac troponin T kinase

  • Paul Pfleiderer
  • Marius P. Sumandea
  • Vitalyi O. Rybin
  • Chaojian Wang
  • Susan F. Steinberg
Original Paper

DOI: 10.1007/s10974-009-9176-y

Cite this article as:
Pfleiderer, P., Sumandea, M.P., Rybin, V.O. et al. J Muscle Res Cell Motil (2009) 30: 67. doi:10.1007/s10974-009-9176-y

Abstract

Phosphorylation of cardiac troponin is a key mechanism involved in regulation of contractile function. In vitro kinase assays revealed that lysates prepared from resting cardiomyocytes contain cardiac troponin I (cTnI) and cTnT kinase activity. cTnI phosphorylation is inhibited by pharmacologic inhibitors of PKA, PKC, Rho kinase and PKC effectors such as RSK and PKD; these kinase inhibitors do not inhibit phosphorylation of cTnT. Rather, cTnT phosphorylation is decreased by the Raf inhibitor GW5074. In vitro kinase assays show that recombinant Raf phosphorylates cTnT, and that Raf-dependent cTnT phosphorylation is abrogated by a T206E substitution; Raf does not phosphorylate cTnI. These studies identify Raf-dependent cTnT-Thr206 phosphorylation as a novel mechanism that would link growth factor-dependent signaling pathways to dynamic changes in cardiac contractile function.

Keywords

Cardiac troponin TRaf-1GW5074CardiomyocytesPhosphorylation

Abbreviations

PKA

Protein kinase A

PKC

Protein kinase C

PKD

Protein kinase D

PDK

Phosphoinositide-dependent protein kinase

ASK-1

Apoptosis signal-regulating kinase-1

RSK

Ribosomal S6 kinase

ROCK

Rho-A-dependent kinase

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Paul Pfleiderer
    • 1
  • Marius P. Sumandea
    • 2
  • Vitalyi O. Rybin
    • 1
  • Chaojian Wang
    • 1
  • Susan F. Steinberg
    • 1
  1. 1.Department of Pharmacology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  2. 2.Department of PhysiologyUniversity of KentuckyLexingtonUSA