Drosophila Rolling pebbles colocalises and putatively interacts with alpha-Actinin and the Sls isoform Zormin in the Z-discs of the sarcomere and with Dumbfounded/Kirre, alpha-Actinin and Zormin in the terminal Z-discs

  • NINA KREISKÖTHER
  • NINA REICHERT
  • DETLEV BUTTGEREIT
  • ALEXANDER HERTENSTEIN
  • KARL-FRIEDRICH FISCHBACH
  • RENATE RENKAWITZ-POHL
Article

DOI: 10.1007/s10974-006-9060-y

Cite this article as:
KREISKÖTHER, N., REICHERT, N., BUTTGEREIT, D. et al. J Muscle Res Cell Motil (2006) 27: 93. doi:10.1007/s10974-006-9060-y

Abstract

The rolling pebbles gene of Drosophila encodes two proteins, one of which, Rols7, is essential for myoblast fusion. In addition, Rols 7 is expressed during myofibrillogenesis and in the mature muscles. Here it overlaps with alpha-Actinin (α-Actn) and the N-terminus of D-Titin/Kettin/Zormin in the Z-line of the sarcomeres. In the attachment sites of the somatic muscles, Rols7 and the immunoglobulin superfamily protein Dumbfounded/Kin of irreC (Duf/Kirre) colocalise. As Duf/Kirre is detectable only transiently, it may be involved in establishing the first contact of the outgrowing muscle fiber to the epidermal attachment site. We propose that Rols7 and Duf/Kirre link the terminal Z-disc to the cell membrane by direct interaction. This is supported by the fact that in yeast two hybrid assays the tetratricopeptide repeat E (TPR E) of Rols7 shows interaction with the intracellular domain of Duf/Kirre. The colocalisation of Rols7 with α-Actn and with D-Titin/Kettin/Zormin in the Z-dics is reflected in␣interactions with different domains of Rols7 in this assay. In summary, these data show that besides the role in myoblast fusion, Rols7 is a scaffold protein during myofibrillogenesis and in the Z-line of the sarcomere as well as in the terminal Z-disc linking the muscle to the epidermal attachment sites.

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • NINA KREISKÖTHER
    • 1
  • NINA REICHERT
    • 1
    • 2
  • DETLEV BUTTGEREIT
    • 1
  • ALEXANDER HERTENSTEIN
    • 3
  • KARL-FRIEDRICH FISCHBACH
    • 3
  • RENATE RENKAWITZ-POHL
    • 1
  1. 1.Fachbereich Biologie, EntwicklungsbiologiePhilipps-Universität MarburgMarburgGermany
  2. 2.Fachbereich Medizin, Institut für Molekularbiologie und TumorforschungPhilipps-Universität MarburgMarburgGermany
  3. 3.Institut für Biologie III (Neurogenetik)Albert-Ludwigs-UniversitätFreiburgGermany