Journal of Muscle Research & Cell Motility

, Volume 26, Issue 6, pp 427–434

Complete human gene structure of obscurin: implications for isoform generation by differential splicing

  • Atsushi Fukuzawa
  • Seraphina Idowu
  • Mathias Gautel
Article

DOI: 10.1007/s10974-005-9025-6

Cite this article as:
Fukuzawa, A., Idowu, S. & Gautel, M. J Muscle Res Cell Motil (2005) 26: 427. doi:10.1007/s10974-005-9025-6

Abstract

The complete gene giant muscle protein obscurin, a modular protein composed largely of tandem Ig-domains, GDP/GTP exchange factor domains (GEF) for small G-proteins, and differentially spliced kinase domains, was analysed. The splice donor and acceptor sites of the 117 exons give important clues for potential splice pathways. The fusion of the conventional obscurin A, containing only the GEF domain, and obscurin B, fusing into the 3′ kinase exons, was experimentally confirmed and analysed. The linker between the two kinases contains multiple predicted phosphorylation sites, as well as a predicted NFX zinc finger domain. Both kinases show only weak homology to either myosin light chain kinases or other giant muscle protein kinases, suggesting that they are functionally distinct.

Copyright information

© Springer 2006

Authors and Affiliations

  • Atsushi Fukuzawa
    • 1
  • Seraphina Idowu
    • 1
  • Mathias Gautel
    • 1
  1. 1.The Randall Division of Cell and Molecular Biophysics and The Cardiovascular DivisionNew Hunt’s House, King’s College LondonLondonUnited Kingdom