Journal of Muscle Research & Cell Motility

, 27:1

TGF-β1 favors the development of fast type identity during soleus muscle regeneration


  • Philippe Noirez
    • CNRS FRE 2412
    • CNRS UMR 7000
    • Université Paris 5
  • Sandra Torres
    • CNRS FRE 2412
  • José Cebrian
    • CNRS FRE 2412
  • Onnik Agbulut
    • CNRS UMR 7000
  • Juliette Peltzer
    • CNRS FRE 2412
  • Gillian Butler-Browne
    • CNRS UMR 7000
  • Dominique Daegelen
    • INSERM U567-CNRS UMR 8104
  • Isabelle Martelly
    • CNRS FRE 2412
  • Angelica Keller
    • CNRS FRE 2412
    • CNRS FRE 2412
    • Laboratoire CRRETCNRS FRE 2412, Faculté de Sciences et Technologies

DOI: 10.1007/s10974-005-9014-9

Cite this article as:
Noirez, P., Torres, S., Cebrian, J. et al. J Muscle Res Cell Motil (2006) 27: 1. doi:10.1007/s10974-005-9014-9


Transforming growth factor-β1 (TGF-β1) is known to be expressed in the environment of developing fast muscle fibres during ontogenesis. In the present study, we have examined effects of administration of either TGF-β1 or neutralizing TGF-β1 antibody on the induction of fast type phenotype in regenerating skeletal muscles in rats. Expressions of fast and slow myosin heavy chain (MHC) isoforms were studied using protein electrophoresis, at 3 and 6 weeks after myotoxic treatment. Muscle contractile properties were also measured in situ. The results have shown that a single injection of TGF-β1 into the regenerating slow soleus muscle increased the expression of fast MHC-2x/d and MHC-2a and decreases that of slow MHC-1 (P<0.05). Moreover, it reduced the degree of tetanic fusion during contraction (P<0.05). Conversely, injection of neutralizing antibody against TGF-β1 into the regenerating fast EDL muscle increased the expression of MHC-2a and MHC-1 (P<0.05). In conclusion, when the slow muscle was regenerating in the presence of an increased level of TGF-β1, it induced a shift to a less slow MHC phenotype and contractile characteristics. Conversely, neutralization of TGF-β1 in the regenerating fast muscle induced a shift to a less fast MHC expression. Together these results suggest that TGF-β1 influences some aspects of fast muscle-type patterning during skeletal muscle regeneration.

Copyright information

© Springer Science+Business Media, Inc. 2005