Journal of Muscle Research & Cell Motility

, Volume 25, Issue 4, pp 337–341

Specificity of blebbistatin, an inhibitor of myosin II

  • John Limouze
  • Aaron F. Straight
  • Timothy Mitchison
  • James R. Sellers
Article

DOI: 10.1007/s10974-004-6060-7

Cite this article as:
Limouze, J., Straight, A.F., Mitchison, T. et al. J Muscle Res Cell Motil (2004) 25: 337. doi:10.1007/s10974-004-6060-7

Abstract

Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. We have examined the specificity and potency of the drug by assaying its effects on the actin-activated MgATPase assay of diverse members of the myosin superfamily. Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Interestingly, smooth muscle which is highly homologous to vertebrate nonmuscle myosin is only poorly inhibited (IC50=80 μM). The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X.

myosinblebbistatininhibitors

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • John Limouze
    • 1
  • Aaron F. Straight
    • 2
  • Timothy Mitchison
    • 2
  • James R. Sellers
    • 1
  1. 1.Laboratory of Molecular Cardiology, National Heart, Lung and Blood InstituteNational Institutes of HealthMD
  2. 2.Institute for Chemistry and Cell Biology, Harvard Medical SchoolMA