Journal of Structural and Functional Genomics

, Volume 14, Issue 2, pp 31–35

Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

  • Michael Soniat
  • Parthasarathy Sampathkumar
  • Garen Collett
  • Anthony S. Gizzi
  • Radhika N. Banu
  • Rahul C. Bhosle
  • Swetha Chamala
  • Sukanya Chowdhury
  • Andras Fiser
  • Alan S. Glenn
  • James Hammonds
  • Brandan Hillerich
  • Kamil Khafizov
  • James D. Love
  • Bridget Matikainen
  • Ronald D. Seidel
  • Rafael Toro
  • P. Rajesh Kumar
  • Jeffery B. Bonanno
  • Yuh Min Chook
  • Steven C. Almo
Article

DOI: 10.1007/s10969-013-9150-1

Cite this article as:
Soniat, M., Sampathkumar, P., Collett, G. et al. J Struct Funct Genomics (2013) 14: 31. doi:10.1007/s10969-013-9150-1

Abstract

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

Keywords

KaryopherinImportinNuclear importNuclear localization signalNucleocytoplasmic transportNuclear poreNab2

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Michael Soniat
    • 1
  • Parthasarathy Sampathkumar
    • 2
  • Garen Collett
    • 1
  • Anthony S. Gizzi
    • 2
  • Radhika N. Banu
    • 2
  • Rahul C. Bhosle
    • 2
  • Swetha Chamala
    • 2
  • Sukanya Chowdhury
    • 2
  • Andras Fiser
    • 2
    • 3
  • Alan S. Glenn
    • 2
  • James Hammonds
    • 2
  • Brandan Hillerich
    • 2
  • Kamil Khafizov
    • 2
    • 3
  • James D. Love
    • 2
  • Bridget Matikainen
    • 2
  • Ronald D. Seidel
    • 2
  • Rafael Toro
    • 2
  • P. Rajesh Kumar
    • 2
  • Jeffery B. Bonanno
    • 2
  • Yuh Min Chook
    • 1
  • Steven C. Almo
    • 2
  1. 1.Department of PharmacologyUniversity of Texas SouthwesternDallasUSA
  2. 2.Department of BiochemistryAlbert Einstein College of MedicineBronxUSA
  3. 3.Department of Systems and Computational BiologyAlbert Einstein College of MedicineBronxUSA