Article

Journal of Structural and Functional Genomics

, Volume 10, Issue 2, pp 165-179

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

The Center for Eukaryotic Structural Genomics

  • John L. MarkleyAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison Email author 
  • , David J. AcetiAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , Craig A. BingmanAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , Brian G. FoxAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , Ronnie O. FrederickAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , Shin-ichi MakinoAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , Karl W. NicholsAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , George N. PhillipsJr.Affiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
  • , John G. PrimmAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
    • , Sarata C. SahuAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
    • , Frank C. VojtikAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
    • , Brian F. VolkmanAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, Medical College of Wisconsin
    • , Russell L. WrobelAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison
    • , Zsolt ZolnaiAffiliated withCenter for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison

Abstract

The Center for Eukaryotic Structural Genomics (CESG) is a “specialized” or “technology development” center supported by the Protein Structure Initiative (PSI). CESG’s mission is to develop improved methods for the high-throughput solution of structures from eukaryotic proteins, with a very strong weighting toward human proteins of biomedical relevance. During the first three years of PSI-2, CESG selected targets representing 601 proteins from Homo sapiens, 33 from mouse, 10 from rat, 139 from Galdieria sulphuraria, 35 from Arabidopsis thaliana, 96 from Cyanidioschyzon merolae, 80 from Plasmodium falciparum, 24 from yeast, and about 25 from other eukaryotes. Notably, 30% of all structures of human proteins solved by the PSI Centers were determined at CESG. Whereas eukaryotic proteins generally are considered to be much more challenging targets than prokaryotic proteins, the technology now in place at CESG yields success rates that are comparable to those of the large production centers that work primarily on prokaryotic proteins. We describe here the technological innovations that underlie CESG’s platforms for bioinformatics and laboratory information management, target selection, protein production, and structure determination by X-ray crystallography or NMR spectroscopy.

Keywords

CESG LIMS PSI Materials Repository Protein Structure Initiative (PSI) PepcDB Plasmid design Protein production Protein structure determination TEV protease