Journal of Structural and Functional Genomics

, Volume 7, Issue 1, pp 37–50

Crystal Structure of the Vitamin B12 Biosynthetic Cobaltochelatase, CbiXS, from Archaeoglobus Fulgidus

  • Jiang Yin
  • Linda X. Xu
  • Maia M. Cherney
  • Evelyne Raux-Deery
  • Amanda A. Bindley
  • Alexei Savchenko
  • John R. Walker
  • Marianne E. Cuff
  • Martin J. Warren
  • Michael N. G. James
ORIGINAL PAPER

DOI: 10.1007/s10969-006-9008-x

Cite this article as:
Yin, J., Xu, L.X., Cherney, M.M. et al. J Struct Funct Genomics (2006) 7: 37. doi:10.1007/s10969-006-9008-x

Abstract

The Archaeoglobus fulgidus gene af0721 encodes CbiXS, a small cobaltochelatase associated with the anaerobic biosynthesis of vitamin B12 (cobalamin). The protein was shown to have activity both in vivo and in vitro, catalyzing the insertion of Co2+ into sirohydrochlorin. The structure of CbiXS was determined in two different crystal forms and was shown to consist of a central mixed β-sheet flanked by four α-helices, one of which originates in the C-terminus of a neighboring molecule. CbiXS is about half the size of other Class II tetrapyrrole chelatases. The overall topography of CbiXS exhibits substantial resemblance to both the N- and C-terminal regions of several members of the Class II metal chelatases involved in tetrapyrrole biosynthesis. Two histidines (His10 and His74), are in similar positions as the catalytic histidine residues in the anaerobic cobaltochelatase CbiK (His145 and His207). In light of the hypothesis that suggests the larger chelatases evolved via gene duplication and fusion from a CbiXS-like enzyme, the structure of AF0721 may represent that of an “ancestral” precursor of class II metal chelatases.

Keywords

Cobalamin (vitamin B12) biosynthesisTetrapyrroleCbiKCbiXCobaltochelataseProtein structure evolution

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Jiang Yin
    • 1
  • Linda X. Xu
    • 2
  • Maia M. Cherney
    • 1
  • Evelyne Raux-Deery
    • 3
  • Amanda A. Bindley
    • 3
  • Alexei Savchenko
    • 2
  • John R. Walker
    • 2
  • Marianne E. Cuff
    • 4
  • Martin J. Warren
    • 3
  • Michael N. G. James
    • 1
    • 5
  1. 1.Group in Protein Structure and Function, Department of BiochemistryUniversity of AlbertaEdmontonCanada
  2. 2.Ontario Centre for Structural ProteomicsC.H. Best Institute, University of TorontoTorontoCanada
  3. 3.Department of BiosciencesUniversity of KentCanterburyUnited Kingdom
  4. 4.Structural Biology Center & Midwest Center for Structural GenomicsBiosciences Division Argonne National LaboratoryArgonneUSA
  5. 5.Alberta Synchrotron InstituteUniversity of AlbertaEdmontonCanada