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The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)

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Abstract

In this work, we analyzed the effects of coffee seed proteins, especially Cc-LTP1 on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-LTP1, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-LTP1 was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-LTP1 has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-LTP1 has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.

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Abbreviations

LTP:

Lipid transfer protein

AMPs:

Antimicrobial peptides

Cc-LTP1 :

Lipid transfer protein from Coffea canephora

FITC:

Fluorescein isothiocyanate

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Acknowledgments

This work is a part of the DS degree thesis of Umberto Zottich Pereira done at Universidade Estadual do Norte Fluminense. We acknowledge financial aid from the Brazilian agencies FAPERJ, CNPq, FAPES and CAPES. We are also thanks for financial aid to Dr. Viviane Veiga do Nascimento through a PNPD/CAPES fellowship.

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Authors and Affiliations

  1. Laboratório de Microbiologia Ambiental e Biotecnologia, Complexo Biopráticas, Universidade Vila Velha, Vila Velha, ES, Brazil

    Umberto Zottich

  2. Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Maura Da Cunha, Germana B. Dias & Guilherme R. Rabelo

  3. Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Antonia Elenir A. Oliveira & Kátia Valevski S. Fernandes

  4. Laboratório de Fisiologia e Bioquímica de Microorganismo, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Umberto Zottich, André O. Carvalho, Viviane V. do Nascimento & Valdirene M. Gomes

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  1. Umberto Zottich
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  2. Maura Da Cunha
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Correspondence to Valdirene M. Gomes.

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Zottich, U., Da Cunha, M., Dias, G.B. et al. The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae). Protein J 33, 422–431 (2014). https://doi.org/10.1007/s10930-014-9575-9

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