The Protein Journal

, Volume 31, Issue 8, pp 651–655

Desferal as Improving Agent for Hemoglobin Fructation: Structural and Functional Impacts

  • Naghmeh Sattarahmady
  • Hossein Heli
  • Ali A. Moosavi-Movahedi
Article

DOI: 10.1007/s10930-012-9444-3

Cite this article as:
Sattarahmady, N., Heli, H. & Moosavi-Movahedi, A.A. Protein J (2012) 31: 651. doi:10.1007/s10930-012-9444-3

Abstract

Hyperglycemia and advanced glycation end products (AGEs) have considerable effects in diabetic patients. So, the recognition of anti-glycation property of compounds has a substantial benefit. Here, desferal, an iron chelator which is one of the most effective drugs in β-thalassemia patients, was chosen to explore its effects on the fructation process of hemoglobin (Hb). The results indicated that desferal had a retardation effect on the functional and structural changes of Hb during fructation. It can prevent the AGE and carbonyl formations and helix depletion during the Hb fructation process. Moreover, desferal can preserve peroxidase and esterase activities of fructated Hb similar as native Hb. Therefore, desferal can be introduced as an anti-glycation drug to prevent the AGE formation.

Keywords

HemoglobinFructoseGlycationDesferal

Abbreviations

AGE

Advanced glycation end products

CD

Circular dichroism

DSC

Differential scanning calorimetry

DNPH

2,4-Dinitrophenylhydrazine

F-Hb

Sample of hemoglobin incubated with 50 mM fructose

F-Hb-D

Sample of hemoglobin incubated with 50 mM fructose +  250 μM desferal

Hb

Hemoglobin

Hb-D

Sample of fresh hemoglobin + 250 μM desferal

p-NPA

p-Nitrophenyl acetate

ROS

Reactive oxygen species

TCA

Trichloroacetic acid

UV

Ultraviolet

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Naghmeh Sattarahmady
    • 1
    • 2
    • 3
  • Hossein Heli
    • 4
  • Ali A. Moosavi-Movahedi
    • 5
  1. 1.Department of Medical Physics, School of MedicineShiraz University of Medical SciencesShirazIran
  2. 2.Department of Nanomedicine, School of Advanced Medical Sciences and TechnologiesShiraz University of Medical SciencesShirazIran
  3. 3.Pharmaceutical Sciences Research Center, School of PharmacyShiraz University of Medical SciencesShirazIran
  4. 4.Department of Chemistry, Science and Research BranchIslamic Azad UniversityFarsIran
  5. 5.Institute of Biochemistry and BiophysicsUniversity of TehranTehranIran