The Protein Journal

, Volume 29, Issue 6, pp 387–393

Towards the Development of Hemerythrin-Based Blood Substitutes

Authors

  • Augustin C. Mot
    • Department of Chemistry and Chemical Engineering“Babes-Bolyai” University
  • Alina Roman
    • Department of Chemistry and Chemical Engineering“Babes-Bolyai” University
  • Iulia Lupan
    • Department of Biology“Babes-Bolyai” University
  • Donald M. KurtzJr
    • Department of ChemistryUniversity of Texas at San Antonio
    • Department of Chemistry and Chemical Engineering“Babes-Bolyai” University
Article

DOI: 10.1007/s10930-010-9264-2

Cite this article as:
Mot, A.C., Roman, A., Lupan, I. et al. Protein J (2010) 29: 387. doi:10.1007/s10930-010-9264-2

Abstract

Hemerythrin is proposed as an alternative to hemoglobin-based blood substitutes. In contrast to hemoglobin, hemerythrin exhibits negligible reactivity towards oxidative and nitrosative stress agents (peroxide, nitric oxide, nitrite). Protocols for attachment of polyethylene glycol and glutaraldehyde cross-linking of Hr are described. These derivatizations appear to have favorable effects on O2 affinity and autoxidation rates for use in blood substitutes. Based on lessons learned from hemoglobin-based blood substitutes, these derivatizations should also help limit extravasation and antigenicity of a hemerythrin-based blood substitute.

Keywords

Hemerythrin Blood substitute Oxygen carrier Hemoglobin

Abbreviations

Hr

Hemerythrin

Hb

Hemoglobin

LB/amp

Luria–Bertani/ampicillin

OD600

Optical density at 600 nm

SDS–PAGE

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

PBS

Phosphate buffer saline

PEG

Polyethelene glycol

MS

Methyl-PEG4-N-hydroxysuccinimide ester

TMS

(Methyl-PEG12)3-PEG4-N-hydroxysuccinimide ester

DMSO

Dimethylsulfoxide

GL

Gluteraldehyde

Copyright information

© Springer Science+Business Media, LLC 2010