Towards the Development of Hemerythrin-Based Blood Substitutes
Hemerythrin is proposed as an alternative to hemoglobin-based blood substitutes. In contrast to hemoglobin, hemerythrin exhibits negligible reactivity towards oxidative and nitrosative stress agents (peroxide, nitric oxide, nitrite). Protocols for attachment of polyethylene glycol and glutaraldehyde cross-linking of Hr are described. These derivatizations appear to have favorable effects on O2 affinity and autoxidation rates for use in blood substitutes. Based on lessons learned from hemoglobin-based blood substitutes, these derivatizations should also help limit extravasation and antigenicity of a hemerythrin-based blood substitute.
KeywordsHemerythrinBlood substituteOxygen carrierHemoglobin
Optical density at 600 nm
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis
Phosphate buffer saline