The Protein Journal

, Volume 29, Issue 2, pp 136–142

Overexpression of Cold Shock Protein A of Psychromonas arctica KOPRI 22215 Confers Cold-Resistance

  • Youn Hong Jung
  • Ji-Yeun Yi
  • Hyun Jung Jung
  • Yoo Kyung Lee
  • Hong Kum Lee
  • Mahendran Chinnamara Naicker
  • Ji-hyun Uh
  • I Seul Jo
  • Eun Jung Jung
  • Hana Im
Article

DOI: 10.1007/s10930-010-9233-9

Cite this article as:
Jung, Y.H., Yi, JY., Jung, H.J. et al. Protein J (2010) 29: 136. doi:10.1007/s10930-010-9233-9

Abstract

A polar bacterium was isolated from Arctic sea sediments and identified as Psychromonas artica, based on 16S rDNA sequence. Psychromonas artica KOPRI 22215 has an optimal growth temperature of 10 °C and a maximum growth temperature of 25 °C, suggesting this bacterium is a psychrophile. Cold shock proteins (Csps) are induced upon temperature downshift by more than 10 °C. Functional studies have researched mostly Csps of a mesophilic bacterium Escherichia coli, but not on those of psychrophilic bacteria. In an effort to understand the molecular mechanisms of psychrophilic bacteria that allow it withstand freezing environments, we cloned a gene encoding a cold shock protein from P. artica KOPRI 22215 (CspAPa) using the conserved sequences in csp genes. The 204 bp-long ORF encoded a protein of 68 amino acids, sharing 56% homology to previously reported E. coli CspA protein. When CspAPa was overexpressed in E. coli, it caused cell growth-retardation and morphological elongation. Interestingly, overexpression of CspAPa drastically increased the host’s cold-resistance by more than ten times, suggesting the protein aids survival in polar environments.

Keywords

Cold-shock protein (Csp)PsychrophileArctic bacteriaCold resistance

Abbreviations

Csp

Cold shock protein

CspAPa

Cold-shock protein A from Psychromonas artica KOPRI 22215

ssDNA

Single-stranded DNA

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Youn Hong Jung
    • 1
  • Ji-Yeun Yi
    • 1
  • Hyun Jung Jung
    • 2
  • Yoo Kyung Lee
    • 2
  • Hong Kum Lee
    • 2
  • Mahendran Chinnamara Naicker
    • 1
  • Ji-hyun Uh
    • 1
  • I Seul Jo
    • 1
  • Eun Jung Jung
    • 1
  • Hana Im
    • 1
  1. 1.Department of Molecular BiologySejong UniversitySeoulKorea
  2. 2.Polar BioCenterKorea Polar Research Institute, Korea Ocean Research and Development InstituteIncheonKorea