Journal of Polymers and the Environment

, Volume 13, Issue 1, pp 39–45

Estimation on Biodegradability of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V) and Numbers of Aerobic PHB/V Degrading Microorganisms in Different Natural Environments

  • Shufang Wang
  • Cunjiang Song
  • Wataru Mizuno
  • Mitsuyo Sano
  • Maeda Maki
  • Chao Yang
  • Banghua Zhang
  • Shigeya Takeuchi
Article

DOI: 10.1007/s10924-004-1214-7

Cite this article as:
Wang, S., Song, C., Mizuno, W. et al. J Polym Environ (2005) 13: 39. doi:10.1007/s10924-004-1214-7

Abstract

To assess the capacity of the natural environment for degrading PHB/V, the film-MPN method proposed previously was modified to estimate the numbers of PHB/V degrading microorganisms (degraders) in various environments. The First-Order Reaction (FOR) model was used to determine the appropriate incubation period for the method. Numbers of aerobic PHB/V degraders were estimated in garden soil, paddy field soil, farm soil, river bank soil, infertile garden soil, river water, activated sludge, and seawater by the film-MPN method. Results were compared with those estimated by the clear-zone technique and showed that the film-MPN method was suitable for estimating the numbers of PHB/V degraders in the environments tested. On the other hand, biodegradability of injection molded PHB/V samples was investigated in several kinds of environments. The changes of weight were studied and results showed that biodegradability of PHB/V related to the numbers of PHB/V degraders in similar ecosystem in different regions. In different environments the biodegradability of PHB/V not only related to the number of PHB/V degraders, but also depended on whether there were conditions for the PHB/V degraders to grow and proliferate easily in the environment.

Keywords

Biodegradationfilm-MPN methodPHB/V degradernatural environmentinjection molded PHB/V sample

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Shufang Wang
    • 1
  • Cunjiang Song
    • 2
  • Wataru Mizuno
    • 3
  • Mitsuyo Sano
    • 4
  • Maeda Maki
    • 4
  • Chao Yang
    • 2
  • Banghua Zhang
    • 1
  • Shigeya Takeuchi
    • 4
  1. 1.State Key Laboratory of Functional Polymer Materials for Adsorption and SeparationInstitute of Polymer Chemistry, Nankai UniversityTianjinP. R. China
  2. 2.Department of Microbiology, Life Science CollegeInstitute of Polymer Chemistry, Nankai UniversityTianjinP. R. China
  3. 3.Toyama Industrial Technology CenterToyama Japan
  4. 4.Faculty of EducationToyama UniversityToyama Japan