Journal of Polymers and the Environment

, Volume 13, Issue 1, pp 39–45

Estimation on Biodegradability of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V) and Numbers of Aerobic PHB/V Degrading Microorganisms in Different Natural Environments

Authors

  • Shufang Wang
    • State Key Laboratory of Functional Polymer Materials for Adsorption and SeparationInstitute of Polymer Chemistry, Nankai University
    • Department of Microbiology, Life Science CollegeInstitute of Polymer Chemistry, Nankai University
  • Wataru Mizuno
    • Toyama Industrial Technology Center
  • Mitsuyo Sano
    • Faculty of EducationToyama University
  • Maeda Maki
    • Faculty of EducationToyama University
  • Chao Yang
    • Department of Microbiology, Life Science CollegeInstitute of Polymer Chemistry, Nankai University
  • Banghua Zhang
    • State Key Laboratory of Functional Polymer Materials for Adsorption and SeparationInstitute of Polymer Chemistry, Nankai University
  • Shigeya Takeuchi
    • Faculty of EducationToyama University
Article

DOI: 10.1007/s10924-004-1214-7

Cite this article as:
Wang, S., Song, C., Mizuno, W. et al. J Polym Environ (2005) 13: 39. doi:10.1007/s10924-004-1214-7

Abstract

To assess the capacity of the natural environment for degrading PHB/V, the film-MPN method proposed previously was modified to estimate the numbers of PHB/V degrading microorganisms (degraders) in various environments. The First-Order Reaction (FOR) model was used to determine the appropriate incubation period for the method. Numbers of aerobic PHB/V degraders were estimated in garden soil, paddy field soil, farm soil, river bank soil, infertile garden soil, river water, activated sludge, and seawater by the film-MPN method. Results were compared with those estimated by the clear-zone technique and showed that the film-MPN method was suitable for estimating the numbers of PHB/V degraders in the environments tested. On the other hand, biodegradability of injection molded PHB/V samples was investigated in several kinds of environments. The changes of weight were studied and results showed that biodegradability of PHB/V related to the numbers of PHB/V degraders in similar ecosystem in different regions. In different environments the biodegradability of PHB/V not only related to the number of PHB/V degraders, but also depended on whether there were conditions for the PHB/V degraders to grow and proliferate easily in the environment.

Keywords

Biodegradationfilm-MPN methodPHB/V degradernatural environmentinjection molded PHB/V sample

Copyright information

© Springer Science+Business Media, Inc. 2005