Journal of Mammary Gland Biology and Neoplasia

, Volume 15, Issue 3, pp 301–318

Extracellular Matrix Composition Reveals Complex and Dynamic Stromal-Epithelial Interactions in the Mammary Gland

Article

DOI: 10.1007/s10911-010-9189-6

Cite this article as:
Maller, O., Martinson, H. & Schedin, P. J Mammary Gland Biol Neoplasia (2010) 15: 301. doi:10.1007/s10911-010-9189-6

Abstract

The mammary gland is an excellent model system to study the interplay between stroma and epithelial cells because of the gland’s unique postnatal development and its distinct functional states. This review focuses on the contribution of the extracellular matrix (ECM) to stromal-epithelial interactions in the mammary gland. We describe how ECM physical properties, protein composition, and proteolytic state impact mammary gland architecture as well as provide instructive cues that influence the function of mammary epithelial cells during pubertal gland development and throughout adulthood. Further, based on recent proteomic analyses of mammary ECM, we describe known mammary ECM proteins and their potential functions, as well as describe several ECM proteins not previously recognized in this organ. ECM proteins are discussed in the context of the morphologically-distinct stromal subcompartments: the basal lamina, the intra- and interlobular stroma, and the fibrous connective tissue. Future studies aimed at in-depth qualitative and quantitative characterization of mammary ECM within these various subcompartments is required to better elucidate the function of ECM in normal as well as in pathological breast tissue.

Keywords

Extracellular matrixStromal-epithelial interactionsBasal laminaCollagenFibronectinLaminin

Abbreviations

collagen IV

type IV collagen

DDR1

discoidin domain receptor 1

EM

electron microscopy

EGFR

epidermal growth factor receptor

ECM

extracellular matrix

ED

extra domain

FACIT

fibril associated collagens with interrupted triple helices

FGF

fibroblast growth factor

FN

fibronectin

GAG

glycosaminoglycan

H&E

hematoxylin and eosin stain

HGF

hepatocyte growth factor

LN

laminin

LAP

latency-associated peptide

LRR

leucine-rich repeats

LOX

lysyl oxidase

MMP

matrix metalloproteinase

MAGP

microfibril-associated glycoprotein

MCP-1

monocyte chemoattractant protein-1

SPARC

secreted protein acidic and rich in cysteine

SLRP

small leucine-rich proteoglycan

SD

Sprague Dawley

TN

tenascin

tTG2

tissue transglutaminase 2

TGF-β

transforming growth factor β

TLR

toll like receptor

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ori Maller
    • 1
    • 2
  • Holly Martinson
    • 1
    • 2
  • Pepper Schedin
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of Medicine, Division of Medical OncologyUniversity of Colorado DenverAuroraUSA
  2. 2.Program in Cancer BiologyUniversity of Colorado DenverAuroraUSA
  3. 3.University of Colorado Cancer CenterAuroraUSA
  4. 4.AMC Cancer Research CenterAuroraUSA