Journal of Mammary Gland Biology and Neoplasia

, Volume 11, Issue 2, pp 151–163

Probing the Microenvironment of Mammary Tumors Using Multiphoton Microscopy

  • Mazen Sidani
  • Jeffrey Wyckoff
  • Chengsen Xue
  • Jeffrey E. Segall
  • John Condeelis
Article

DOI: 10.1007/s10911-006-9021-5

Cite this article as:
Sidani, M., Wyckoff, J., Xue, C. et al. J Mammary Gland Biol Neoplasia (2006) 11: 151. doi:10.1007/s10911-006-9021-5

Abstract

Advances in optical imaging technologies that allow the subcellular resolution of undissected tissue have begun to offer new clues into the biology of development and disease. For cancer, such advances mean that the primary tumor is no longer a black box and that the disease can be studied throughout the metastatic cascade and not just as an endpoint. In this review we examine the advances in multiphoton imaging technology that have been used to define the microenvironment and its role in delineating the invasion and intravasation steps of metastasis inside living mammary tumors. Results show that the tumor microenvironment is a dynamic place where interactions between tumor cells, macrophages, blood vessels, and extracellular matrix fibers define the metastatic phenotype.

Keywords

Intravital imagingGFPReal-time imagingBreast cancerSecond harmonic generation

Abbreviations

ECM

extracellular matrix

MPIVI

multiphoton-based intravital imaging

SHG

second harmonic generation

MMPs

matrix metallo-proteases

GFP

green fluorescent protein

CFP

cyan fluorescent protein

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Mazen Sidani
    • 1
  • Jeffrey Wyckoff
    • 1
    • 2
  • Chengsen Xue
    • 1
  • Jeffrey E. Segall
    • 1
  • John Condeelis
    • 1
    • 2
  1. 1.Department of Anatomy and Structural BiologyAlbert Einstein College of Medicine of Yeshiva UniversityBronxUSA
  2. 2.Analytical Imaging FacilityAlbert Einstein College of Medicine of Yeshiva UniversityBronxUSA