Skip to main content
SpringerLink
Log in

Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction with 7-Hydroxycoumarin

  • SHORT COMMUNICATION
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

A novel, simple, selective and sensitive spectrofluorimetric method was developed and validated for the determination of perindopril erbumine using 7-hydroxycoumarin. Perindopril erbumine was found to react with 7-hydroxycoumarin in acetonitrile resulting in a new fluorescent product with about 58 nm blue shifted emission. The fluorescence of the complex was measured at 440 nm after excitation at 350 nm in acetonitrile. Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 2.0–16.0 μg/mL (R2 = 1) with a detection limit of 0.054 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the proposed and reference method revealed no significant differences in the performance of the two methods regarding the accuracy and precision respectively. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. A proposal for the reaction pathway with 7-hydroxycoumarin was postulated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Scheme 1
Fig. 7

References

  1. Committee JF (2014) British national formulary, 67th edn. British Medical Association and Royal Pharmaceutical Society of Great Britain, London

    Google Scholar 

  2. Raju VB, Rao AL (2011) Development and validation of new HPLC method for the estimation of perindopril in tablet dosage forms. Rasayan J Chem 4:113–116

    CAS  Google Scholar 

  3. Zaazaa HE, Abbas SS, MEssam HA, El Bardicy MG (2013) Validated chromatographic methods for determination of perindopril and amlodipine in pharmaceutical formulation in the presence of their degradation products. Sci Math J Chromatogr Sci 51:533–543

    Article  CAS  Google Scholar 

  4. Riyaz SMD, Vasanth PM, Ramesh M, Ramesh R, Ramesh T (2012) A sensitive RP-HPLC method development and validation for the simultaneous estimation of perindopril erbuminee and amlodipine besilate in tablet dosage form. Int J Chem Life Sci 1:1033–1038

    Google Scholar 

  5. Simončič Z, Roškar R, Gartner A, Kogej K, Kmetec V (2008) The use of microcalorimetry and HPLC for the determination of degradation kinetics and thermodynamic parameters of Perindopril Erbuminee in aqueous solutions. Int J Pharm 356:200–205

    Article  PubMed  Google Scholar 

  6. Chaudhary AB, Patel RK, Chaudhary SA (2010) Determination of losartan potassium and perindopril erbumine in tablet formulations by reversed-phase HPLC. Int J Chem Tech Res 2:1141–1146

    CAS  Google Scholar 

  7. Jogia H, Khandelwal U, Gandhi T, Singh S, Modi D (2010) Development and validation of a stability-indicating assay method for simultaneous determination of perindopril and indapamide in combined dosage form by reversed-phase high-performance liquid chromatography. J AOAC Int 93:108–115

    CAS  PubMed  Google Scholar 

  8. Joseph J, Philip B, Sundarapandian M (2011) Method development and validation for simultaneous estimation of perindopril erbumine and indapamide by RP-HPLC in pharmaceutical dosage forms. Int J Pharm Pharm Sci 3:288–293

    CAS  Google Scholar 

  9. Prajapati J, Patel A, Patel MB, Prajapati N, Prajapati R (2011) Analytical method development and validation of amlodipine besylate and perindopril erbuminee in combine dosage form by RP-HPLC. Int J PharmTech Res 3:801–808

    CAS  Google Scholar 

  10. Jaina DS, Subbaiah G, Sanya M, Pande UC, Shrivastav P (2006) First LC–MS/MS electrospray ionization validated method for the quantification of perindopril and its metabolite perindoprilat in human plasma and its application to bioequivalence study. J Chromatogr B 837:92–100

    Article  Google Scholar 

  11. Nirogi RVS, Kandikere VN, Shukla M, Mudigonda K, Maurya S, Komarneni P (2006) High-throughput quantification of perindopril in human plasma by liquid chromatography/tandem mass spectrometry: application to a bioequivalence study. Rapid Commun Mass Spectrom 20:1864–1870

    Article  CAS  PubMed  Google Scholar 

  12. Dewani MG, Bothara KG, Madgulkar AR, Damle MC (2011) Simultaneous estimation of perindopril erbuminee and indapamide in bulk drug and tablet dosage form by HPTLC. Int J Comprehen Pharm 2:1–4

    Google Scholar 

  13. Neelam S, Gopisetti J, Chandra BS (2012) Spectrophotometric analysis of perindopril erbumine in bulk and tablets using bromophenol blue. Der Pharm Let 4:159–169

    Google Scholar 

  14. Rahman N, Rahman H, Khatoon A (2012) Development of spectrophotometric method for the determination of perindopril erbuminee in pharmaceutical formulations using 2, 4 dinitrofluorobenzene. J Chil Chem Soc 57:1069–1073

    Article  CAS  Google Scholar 

  15. Prajapati J, Patel MB, Prajapati N, Prajapati R (2011) Simultaneous determination of perindopril erbumine and amlodipine besylate by absorption factor method. Int J Applied Biol Pharm Technol 2:230–233

    Google Scholar 

  16. Sharma S, Sharma MC (2011) UV-Spectrophotometric method for the perindopril erbumine in pharmaceutical formulations using indigo carmine. Am-Eur J Scientif Res 6:210–216

    CAS  Google Scholar 

  17. Hermanson GT (2013) In: Bioconjugate Techniques (ed) Academic press p.418

  18. Stewart JT, Lotti DM (1971) Fluorometric determination of amphetamines with 3-carboxy-7-hydroxycoumarin. J Pharm Sci 60:461–463

    Article  CAS  PubMed  Google Scholar 

  19. Harvey D (2009) Modern analytical chemistry (ed) David Harvey

  20. International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use (2005) Validation of Analytical Procedures: Text and Methodology ICH Q2 (R1)

Download references

Funding

This study was funded by University of Aleppo, Aleppo, Syria.

Conflict of Interest

The authors declare that they have no conflict of interest.

Authors’ Contributions

Hanan Fael has performed the experimental and analytical work and prepared the draft of the manuscript. The supervision of this work was provided by Prof. Amir Al-Haj Sakur. All authors read and approved the final manuscript.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo, Syrian Arab Republic

    Hanan Fael & Amir Al-Haj Sakur

Authors
  1. Hanan Fael
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amir Al-Haj Sakur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hanan Fael.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fael, H., Sakur, A.AH. Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction with 7-Hydroxycoumarin. J Fluoresc 25, 811–818 (2015). https://doi.org/10.1007/s10895-015-1596-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-015-1596-z

Keywords

Search

Navigation