Journal of Fluorescence

, Volume 21, Issue 3, pp 1223–1230

Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe

Authors

  • Carolina D. Andrade
    • Department of ChemistryUniversity of Central Florida
  • Ciceron O. Yanez
    • Department of ChemistryUniversity of Central Florida
  • Maher A. Qaddoura
    • Department of ChemistryUniversity of Central Florida
  • Xuhua Wang
    • Department of ChemistryUniversity of Central Florida
  • Curtesa L. Arnett
    • Department of ChemistryUniversity of Central Florida
  • Sabrina A. Coombs
    • Department of ChemistryUniversity of Central Florida
  • Jin Yu
    • Department of ChemistryUniversity of Central Florida
  • Rania Bassiouni
    • Department of ChemistryUniversity of Central Florida
  • Mykhailo V. Bondar
    • Department of PhotoactivityInstitute of Physics
    • Department of ChemistryUniversity of Central Florida
    • CREOL, The College of Optics and PhotonicsUniversity of Central Florida
Original Paper

DOI: 10.1007/s10895-010-0801-3

Cite this article as:
Andrade, C.D., Yanez, C.O., Qaddoura, M.A. et al. J Fluoresc (2011) 21: 1223. doi:10.1007/s10895-010-0801-3

Abstract

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

Keywords

Fluorescent dyesPluronicMultiphoton absorptionBioimagingFluorescence microscopyLysosomes

Copyright information

© Springer Science+Business Media, LLC 2011