Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe
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- Andrade, C.D., Yanez, C.O., Qaddoura, M.A. et al. J Fluoresc (2011) 21: 1223. doi:10.1007/s10895-010-0801-3
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We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.