Journal of Fluorescence

, Volume 21, Issue 2, pp 707–714

Photobleaching on Photonic Crystal Enhanced Fluorescence Surfaces

  • Vikram Chaudhery
  • Meng Lu
  • Cheng Sheng Huang
  • Sherine George
  • Brian T. Cunningham
Original Paper

DOI: 10.1007/s10895-010-0760-8

Cite this article as:
Chaudhery, V., Lu, M., Huang, C.S. et al. J Fluoresc (2011) 21: 707. doi:10.1007/s10895-010-0760-8

Abstract

The effect of resonant fluorescent enhancement from a photonic crystal surface upon the fluorescent photobleaching rate of Cyanine-5 labeled protein has been investigated. We show that the enhanced excitation mechanism for photonic crystal enhanced fluorescence, in which the device surface resonantly couples light from an excitation laser, accelerates photobleaching in proportion to the coupling efficiency of the laser to the photonic crystal. We also show that the enhanced extraction mechanism, in which the photonic crystal directs emitted photons approximately normal to the surface, does not play a role in the rate of photobleaching. We show that the photobleaching rate of dye molecules on the photonic crystal surface is accelerated by 30x compared to an ordinary glass surface, but substantial signal gain is still evident, even after extended periods of continuous illumination at the resonant condition.

Keywords

Photonic crystal Enhanced fluorescence Photobleaching 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Vikram Chaudhery
    • 1
  • Meng Lu
    • 3
  • Cheng Sheng Huang
    • 1
  • Sherine George
    • 2
  • Brian T. Cunningham
    • 1
    • 2
  1. 1.Department of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of BioengineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.SRU BiosystemsWoburnUSA

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