Journal of Fluorescence

, Volume 20, Issue 6, pp 1307–1313

A Highly Selective Fluorescent Probe for Detection of Biological Samples Thiol and Its Application in Living Cells

Authors

  • Qing-Ping Zuo
    • Pharmaceutics Department, College of pharmacyCentral South University
    • Department of Pharmacy, The Third Xiangya HospitalCentral South University
  • Bing Li
    • Department of Pharmacy, The Third Xiangya HospitalCentral South University
  • Qi Pei
    • Department of Pharmacy, The Third Xiangya HospitalCentral South University
  • Zuojun Li
    • Department of Pharmacy, The Third Xiangya HospitalCentral South University
    • Department of Pharmacy, The Third Xiangya HospitalCentral South University
    • Pharmaceutics Department, College of pharmacyCentral South University
Short Communication

DOI: 10.1007/s10895-010-0673-6

Cite this article as:
Zuo, Q., Li, B., Pei, Q. et al. J Fluoresc (2010) 20: 1307. doi:10.1007/s10895-010-0673-6

Abstract

Probe 1 was designed and synthesized as a new fluorescent molecular probe for thiols in PBS buffer at physiological condition. This fluorescent molecular probe consists of a thiol reaction moiety bound to a coumarin fluorophore. Its fluorescence quantum yield is low, but a drastic enhancement of fluorescence intensity was observed in the presence of thiols. Possible interference with other analytes was examined. Probe 1 displays a highly selective fluorescent enhancement with thiols, and the probe was successfully applied to thiols determination in intracellular, in human urine and blood samples.

Keywords

FluorescenceThiolsSensingFluorescent probe

Copyright information

© Springer Science+Business Media, LLC 2010