Journal of Fluorescence

, Volume 20, Issue 6, pp 1307–1313

A Highly Selective Fluorescent Probe for Detection of Biological Samples Thiol and Its Application in Living Cells

  • Qing-Ping Zuo
  • Bing Li
  • Qi Pei
  • Zuojun Li
  • Shi-Kun Liu
Short Communication

DOI: 10.1007/s10895-010-0673-6

Cite this article as:
Zuo, Q., Li, B., Pei, Q. et al. J Fluoresc (2010) 20: 1307. doi:10.1007/s10895-010-0673-6

Abstract

Probe 1 was designed and synthesized as a new fluorescent molecular probe for thiols in PBS buffer at physiological condition. This fluorescent molecular probe consists of a thiol reaction moiety bound to a coumarin fluorophore. Its fluorescence quantum yield is low, but a drastic enhancement of fluorescence intensity was observed in the presence of thiols. Possible interference with other analytes was examined. Probe 1 displays a highly selective fluorescent enhancement with thiols, and the probe was successfully applied to thiols determination in intracellular, in human urine and blood samples.

Keywords

FluorescenceThiolsSensingFluorescent probe

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Qing-Ping Zuo
    • 2
    • 1
  • Bing Li
    • 1
  • Qi Pei
    • 1
  • Zuojun Li
    • 1
  • Shi-Kun Liu
    • 1
    • 2
  1. 1.Department of Pharmacy, The Third Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China
  2. 2.Pharmaceutics Department, College of pharmacyCentral South UniversityChangshaPeople’s Republic of China
  3. 3.Pharmaceutics Department, College of pharmacyCentral South UniversityChangshaPeople’s Republic of China
  4. 4.Department of Pharmacy, The Third Xiangya HospitalCentral South UniversityChangshaChina