Journal of Fluorescence

, 19:381

Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation

Rapid Communication

DOI: 10.1007/s10895-008-0405-3

Cite this article as:
De, A.K. & Goswami, D. J Fluoresc (2009) 19: 381. doi:10.1007/s10895-008-0405-3


We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.


Two-photon cross-sectionPhoto-damageFluorescence enhancementMultiphoton imaging

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of ChemistryIndian Institute of Technology KanpurKanpurIndia