, Volume 19, Issue 2, pp 381-386
Date: 21 Aug 2008

Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation

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We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.