Journal of Fluorescence

, Volume 18, Issue 6, pp 1151–1155

Nonlinear Excitation of Tryptophan Emission Enhanced by Silver Nanoparticles

  • Diego Rativa
  • Anderson S. L. Gomes
  • Sebastian Wachsmann-Hogiu
  • Daniel L. Farkas
  • Renato E. de Araujo
Original Paper

DOI: 10.1007/s10895-008-0366-6

Cite this article as:
Rativa, D., Gomes, A.S.L., Wachsmann-Hogiu, S. et al. J Fluoresc (2008) 18: 1151. doi:10.1007/s10895-008-0366-6

Abstract

We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.

Keywords

TryptophanAutofluorescenceMetallic nanoparticlesNonlinear excitation

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Diego Rativa
    • 1
  • Anderson S. L. Gomes
    • 2
  • Sebastian Wachsmann-Hogiu
    • 3
    • 4
  • Daniel L. Farkas
    • 3
  • Renato E. de Araujo
    • 1
  1. 1.Department of Electronic and SystemsUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Department of PhysicsUniversidade Federal de PernambucoRecifeBrazil
  3. 3.Minimally Invasive Surgical Technologies Institute and Department of SurgeryCedars-Sinai Medical CenterLos AngelesUSA
  4. 4.NSF Center for Biophotonics Science and TechnologyUniversity of California DavisSacramentoUSA