Original Paper

Journal of Fluorescence

, Volume 17, Issue 6, pp 727-738

First online:

Single-molecule Detection of Reactive Oxygen Species: Application to Photocatalytic Reactions

  • Takashi TachikawaAffiliated withThe Institute of Scientific and Industrial Research (SANKEN), Osaka University
  • , Tetsuro MajimaAffiliated withThe Institute of Scientific and Industrial Research (SANKEN), Osaka University Email author 

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In this review, we have focused on the oxidation reactions of single dye molecules by reactive oxygen species (ROS). The methodologies for the single-molecule detection of ROS, such as hydroxyl radical (HO), singlet oxygen (O2(a1Δg)), and hydrogen peroxide (H2O2), have been introduced together with examples. In particular, a successful application using the single-molecule fluorescence technique for the investigation of the TiO2 photocatalytic oxidation reactions is demonstrated in detail.


Single-molecule fluorescence spectroscopy Reactive oxygen species TiO2 photocatalyst Photooxidation